ABCC1 p.Lys267Met
[switch to full view]Comments [show]
None has been submitted yet.
PMID: 11301332
[PubMed]
Ren XQ et al: "Glutathione-dependent binding of a photoaffinity analog of agosterol A to the C-terminal half of human multidrug resistance protein."
No.
Sentence
Comment
91
The primers used to introduce two mutations, K267M and W261A, into MRP1 were 5Ј-GAGTGCGCCAT- GACTAGGAAG-3Ј (forward) and 5Ј-CTTCTTCGCGTTCTTTACCA- AAAC-3Ј (reverse) encoding mismatched bases (bold).
X
ABCC1 p.Lys267Met 11301332:91:45
status: NEW
PMID: 16098482
[PubMed]
Noguchi T et al: "MRP1 mutated in the L0 region transports SN-38 but not leukotriene C4 or estradiol-17 (beta-D-glucuronate)."
No.
Sentence
Comment
60
In order to completely disrupt LTC4 transport activity, we created additional mutations in the L0 region of MRP1 that already expressed the W261A and K267M mutations.
X
ABCC1 p.Lys267Met 16098482:60:150
status: NEW63 Practically, the W261A and K267M mutations were first introduced into wt MRP1 by PCR using primers described in a previous paper [15].
X
ABCC1 p.Lys267Met 16098482:63:27
status: NEW136 As described in Section 2, the new mutant MRP1, named dmL0MRP1, was designed to carry the extra mutations, W222L, W223L and R230A, in addition to the previous mutants, W261A and K267M, in L0 region, and the original TMD0 region, in order to avoid the influence of deletion of the TMD0 region (Fig. 1).
X
ABCC1 p.Lys267Met 16098482:136:178
status: NEW
PMID: 17187268
[PubMed]
Bakos E et al: "Portrait of multifaceted transporter, the multidrug resistance-associated protein 1 (MRP1/ABCC1)."
No.
Sentence
Comment
97
Typical substrates, which are transported as glutathione or glucuronide conjugates, e.g., LTC4 or E217βG, are not transported by an MRP1 variant mutated in the L0 region (W222L, W223L, R231A, W261A, K267M).
X
ABCC1 p.Lys267Met 17187268:97:205
status: NEW
PMID: 17110465
[PubMed]
Vila-Carriles WH et al: "Defining a binding pocket for sulfonylureas in ATP-sensitive potassium channels."
No.
Sentence
Comment
100
Additionally, it was observed that GSH-dependent photoaffinity labeling of MRP1 requires the L0 linker and a double mutation, W261A and K267M, in L0 decreased labeling three-fold (35).
X
ABCC1 p.Lys267Met 17110465:100:136
status: NEW99 Additionally, it was observed that GSH-dependent photoaffinity labeling of MRP1 requires the L0 linker and a double mutation, W261A and K267M, in L0 decreased labeling three-fold (35).
X
ABCC1 p.Lys267Met 17110465:99:136
status: NEW