ABCMdb

PMID: 17110465

Vila-Carriles WH, Zhao G, Bryan J
Defining a binding pocket for sulfonylureas in ATP-sensitive potassium channels.
FASEB J. 2007 Jan;21(1):18-25. Epub 2006 Nov 16., [PubMed]

Sentences

No. Mutations Sentence Comment

99 ABCC1 p.Trp261Ala ABCC1 p.Lys267Met Additionally, it was observed that GSH-dependent photoaffinity labeling of MRP1 requires the L0 linker and a double mutation, W261A and K267M, in L0 decreased labeling three-fold (35). Login to comment 100 ABCC1 p.Trp261Ala ABCC1 p.Lys267Met Additionally, it was observed that GSH-dependent photoaffinity labeling of MRP1 requires the L0 linker and a double mutation, W261A and K267M, in L0 decreased labeling three-fold (35). Login to comment 103 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala The graph shows a decrease in photoaffinity labeling for SUR1 Y230A and W232A (Fig. 3C). Login to comment 104 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala The graph shows a decrease in photoaffinity labeling for SUR1 Y230A and W232A (Fig. 3C). Login to comment 125 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala The data are plotted as the ratio of label-to- protein in arbitrary units afe; sd. the SUR1, Y230A, and W232A substitutions. Login to comment 126 ABCC8 p.Trp232Ala ABCC8 p.Trp232Ala ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala ABCC8 p.Tyr230Ala ABCC8 p.Tyr230Ala The data are plotted as the ratio of label-toprotein in arbitrary units Ϯ sd. the SUR1, Y230A, and W232A substitutions. Login to comment 127 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala Both SUR1 and KIR6.2 in KATP channels are affinity-labeled by [125 I]-azido-glibenclamide and coexpression of SUR1, Y230A, and W232A with KIR6.2 resulted in labeling of the KIR (data not shown) and also in an increase of the mutant SUR1 labeling (Fig. 6). Login to comment 128 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala When SUR1 Y230A, and W232A were coexpressed with टèc;33KIR6.2, a KIR6.2 construct that does not photolabel, none of the SUR1 mutants labeled (Fig. 6), indicating that the KIR6.2 N terminus play a role in the sulfonylurea binding pocket. Login to comment 129 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala When SUR1 Y230A, and W232A were coexpressed with ⌵⌬33KIR6.2, a KIR6.2 construct that does not photolabel, none of the SUR1 mutants labeled (Fig. 6), indicating that the KIR6.2 N terminus play a role in the sulfonylurea binding pocket. Login to comment 183 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala When SUR1 and the SUR1 alanine point mutations Y230A and W232A were coexpressed with KIR, this resulted in an increase in [125 I]-azido-glibenclamide labeling and KIR cophotolabeling, consistent with KIR forming part of the B site. Login to comment 184 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala When SUR1 and the SUR1 alanine point mutations Y230A and W232A were coexpressed with KIR, this resulted in an increase in [125 I]-azido-glibenclamide labeling and KIR cophotolabeling, consistent with KIR forming part of the B site. Login to comment 188 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala Wild-type SUR1, SUR1, Y230A, and W232A were expressed or coexpressed with either KIR6.2 or Nèc;33KIR6.2 in COSm6 cells. Login to comment 189 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala Wild-type SUR1, SUR1, Y230A, and W232A were expressed or coexpressed with either KIR6.2 or N⌬33KIR6.2 in COSm6 cells. Login to comment 191 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala However, coexpression of SUR1, Y230A, and W232A with Nèc;33KIR6.2 did not increase labeling of SUR1 or the mutants. Login to comment 192 ABCC8 p.Trp232Ala ABCC8 p.Tyr230Ala However, coexpression of SUR1, Y230A, and W232A with N⌬33KIR6.2 did not increase labeling of SUR1 or the mutants. Login to comment