ABCMdb

PMID: 16098482

Noguchi T, Ren XQ, Aoki S, Igarashi Y, Che XF, Nakajima Y, Takahashi H, Mitsuo R, Tsujikawa K, Sumizawa T, Haraguchi M, Kobayashi M, Goto S, Kanehisa M, Aikou T, Akiyama S, Furukawa T
MRP1 mutated in the L0 region transports SN-38 but not leukotriene C4 or estradiol-17 (beta-D-glucuronate).
Biochem Pharmacol. 2005 Oct 1;70(7):1056-65., 2005-10-01 [PubMed]

Sentences

No. Mutations Sentence Comment

4 ABCC1 p.Trp222Leu ABCC1 p.Trp223Leu ABCC1 p.Arg230Ala In this paper, we further tested the effect of W222L, W223L and R230A mutations in MRP1, designated dmL0MRP1, on MRP1 transport activity. Login to comment 60 ABCC1 p.Trp261Ala ABCC1 p.Lys267Met In order to completely disrupt LTC4 transport activity, we created additional mutations in the L0 region of MRP1 that already expressed the W261A and K267M mutations. Login to comment 61 ABCC1 p.Trp222Leu ABCC1 p.Trp223Leu ABCC1 p.Arg230Ala The extra mutations created, W222L, W223L and R230A, were located within WxxxxxK sequences in the L0 region that have been reported to an interacting site of glutathione-S-transferase with GSH. Login to comment 63 ABCC1 p.Trp261Ala ABCC1 p.Lys267Met Practically, the W261A and K267M mutations were first introduced into wt MRP1 by PCR using primers described in a previous paper [15]. Login to comment 64 ABCC1 p.Trp222Leu ABCC1 p.Trp223Leu ABCC1 p.Arg230Ala W222L, W223L and R230A mutations were then introduced into this construct by PCR using the forward primers, 50 -GGGTTGATTGTCGCGGGCTACCGCC-30 , and reverse primer 50 -TGTGATCAACAAGAAGGTGATCC- TC-30 (bold denotes mismatched bases encoding the mutation). Login to comment 136 ABCC1 p.Trp261Ala ABCC1 p.Lys267Met ABCC1 p.Trp222Leu ABCC1 p.Trp223Leu ABCC1 p.Arg230Ala As described in Section 2, the new mutant MRP1, named dmL0MRP1, was designed to carry the extra mutations, W222L, W223L and R230A, in addition to the previous mutants, W261A and K267M, in L0 region, and the original TMD0 region, in order to avoid the influence of deletion of the TMD0 region (Fig. 1). Login to comment