PMID: 11301332

Ren XQ, Furukawa T, Aoki S, Nakajima T, Sumizawa T, Haraguchi M, Chen ZS, Kobayashi M, Akiyama S
Glutathione-dependent binding of a photoaffinity analog of agosterol A to the C-terminal half of human multidrug resistance protein.
J Biol Chem. 2001 Jun 22;276(25):23197-206. Epub 2001 Apr 11., 2001-06-22 [PubMed]
Sentences
No. Mutations Sentence Comment
91 ABCC1 p.Trp261Ala
X
ABCC1 p.Trp261Ala 11301332:91:55
status: NEW
view ABCC1 p.Trp261Ala details
ABCC1 p.Lys267Met
X
ABCC1 p.Lys267Met 11301332:91:45
status: NEW
view ABCC1 p.Lys267Met details
 The primers used to introduce two mutations, K267M and W261A, into MRP1 were 5Ј-GAGTGCGCCAT- GACTAGGAAG-3Ј (forward) and 5Ј-CTTCTTCGCGTTCTTTACCA- AAAC-3Ј (reverse) encoding mismatched bases (bold). Login to comment 255 ABCC1 p.Trp261Ala
X
ABCC1 p.Trp261Ala 11301332:255:32
status: NEW
view ABCC1 p.Trp261Ala details
ABCC1 p.Lys267Ala
X
ABCC1 p.Lys267Ala 11301332:255:43
status: NEW
view ABCC1 p.Lys267Ala details
 This was tested by substituting Trp261 and Lys267 with Ala and Met, respectively, and the subsequent mutated L0 region tested in binding studies. Login to comment