ABCC1 p.Trp261Ala

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PMID: 11301332 [PubMed] Ren XQ et al: "Glutathione-dependent binding of a photoaffinity analog of agosterol A to the C-terminal half of human multidrug resistance protein."
No. Sentence Comment
91 The primers used to introduce two mutations, K267M and W261A, into MRP1 were 5Ј-GAGTGCGCCAT- GACTAGGAAG-3Ј (forward) and 5Ј-CTTCTTCGCGTTCTTTACCA- AAAC-3Ј (reverse) encoding mismatched bases (bold).
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ABCC1 p.Trp261Ala 11301332:91:55
status: NEW
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255 This was tested by substituting Trp261 and Lys267 with Ala and Met, respectively, and the subsequent mutated L0 region tested in binding studies.
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ABCC1 p.Trp261Ala 11301332:255:32
status: NEW
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PMID: 16098482 [PubMed] Noguchi T et al: "MRP1 mutated in the L0 region transports SN-38 but not leukotriene C4 or estradiol-17 (beta-D-glucuronate)."
No. Sentence Comment
60 In order to completely disrupt LTC4 transport activity, we created additional mutations in the L0 region of MRP1 that already expressed the W261A and K267M mutations.
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ABCC1 p.Trp261Ala 16098482:60:140
status: NEW
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63 Practically, the W261A and K267M mutations were first introduced into wt MRP1 by PCR using primers described in a previous paper [15].
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ABCC1 p.Trp261Ala 16098482:63:17
status: NEW
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136 As described in Section 2, the new mutant MRP1, named dmL0MRP1, was designed to carry the extra mutations, W222L, W223L and R230A, in addition to the previous mutants, W261A and K267M, in L0 region, and the original TMD0 region, in order to avoid the influence of deletion of the TMD0 region (Fig. 1).
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ABCC1 p.Trp261Ala 16098482:136:168
status: NEW
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PMID: 17187268 [PubMed] Bakos E et al: "Portrait of multifaceted transporter, the multidrug resistance-associated protein 1 (MRP1/ABCC1)."
No. Sentence Comment
97 Typical substrates, which are transported as glutathione or glucuronide conjugates, e.g., LTC4 or E217βG, are not transported by an MRP1 variant mutated in the L0 region (W222L, W223L, R231A, W261A, K267M).
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ABCC1 p.Trp261Ala 17187268:97:198
status: NEW
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PMID: 17110465 [PubMed] Vila-Carriles WH et al: "Defining a binding pocket for sulfonylureas in ATP-sensitive potassium channels."
No. Sentence Comment
100 Additionally, it was observed that GSH-dependent photoaffinity labeling of MRP1 requires the L0 linker and a double mutation, W261A and K267M, in L0 decreased labeling three-fold (35).
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ABCC1 p.Trp261Ala 17110465:100:126
status: NEW
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99 Additionally, it was observed that GSH-dependent photoaffinity labeling of MRP1 requires the L0 linker and a double mutation, W261A and K267M, in L0 decreased labeling three-fold (35).
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ABCC1 p.Trp261Ala 17110465:99:126
status: NEW
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