ABCB2 p.Gln277Cys
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PMID: 21540052
[PubMed]
Pinto RD et al: "Transporters associated with antigen processing (TAP) in sea bass (Dicentrarchus labrax, L.): molecular cloning and characterization of TAP1 and TAP2."
No.
Sentence
Comment
161
Mutations on highly conserved residues of CH1/CL1 (except Q277C) and of sensor region (G282C, I284C, R287C) interfered with and drastically affected transport, respectively.
X
ABCB2 p.Gln277Cys 21540052:161:58
status: NEW162 Mutations on highly conserved residues of CH1/CL1 (except Q277C) and of sensor region (G282C, I284C, R287C) interfered with and drastically affected transport, respectively.
X
ABCB2 p.Gln277Cys 21540052:162:58
status: NEW
PMID: 19297616
[PubMed]
Oancea G et al: "Structural arrangement of the transmission interface in the antigen ABC transport complex TAP."
No.
Sentence
Comment
66
Remarkably, all mutations in coupling helix 1, except for Q277C, interfered with the transport activity.
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ABCB2 p.Gln277Cys 19297616:66:58
status: NEW129 We chose 2 pairs of mutations, Q277C/E602C and A381C/E602C, containing a single cysteine in each coupling helix of TAP1.
X
ABCB2 p.Gln277Cys 19297616:129:31
status: NEW132 Surprisingly, cross-linking of Q277C/E602C arrested TAP in a translocation-incompetent state, in which peptide binding was unaffected (Fig. 5C).
X
ABCB2 p.Gln277Cys 19297616:132:31
status: NEW133 The transport activity of Cys-less/E602C is only slightly reduced due to irreversible oxidation.
X
ABCB2 p.Gln277Cys 19297616:133:31
status: NEW67 Remarkably, all mutations in coupling helix 1, except for Q277C, interfered with the transport activity.
X
ABCB2 p.Gln277Cys 19297616:67:58
status: NEW130 We chose 2 pairs of mutations, Q277C/E602C and A381C/E602C, containing a single cysteine in each coupling helix of TAP1.
X
ABCB2 p.Gln277Cys 19297616:130:31
status: NEW135 Surprisingly, cross-linking of Q277C/E602C arrested TAP in a translocation-incompetent state, in which peptide binding was unaffected (Fig. 5C).
X
ABCB2 p.Gln277Cys 19297616:135:31
status: NEW
PMID: 17164240
[PubMed]
Herget M et al: "Mechanism of substrate sensing and signal transmission within an ABC transporter: use of a Trojan horse strategy."
No.
Sentence
Comment
39
Expression of Human TAP Mutants-The single cysteine TAP1 mutants, Q277C, G282C, N283C, I284C, M285C, S286C, R287C, V288C, and R659C, were generated by ligase chain reaction with the following primers using cysteine-less human TAP1 with a C-terminal His10 tag as template (16): Q277C, CCGAATTCTTCCAGTGCAACCAGACCGC; G282C, GCA- GAACCAGACCTGCAACATCATGTCC; N283C, CAGAAC- CAGACCGGCTGCATCATGTCCAGAG; I284C, GACCGG- CAACTGCATGTCCAGAG; M285C, GACCGGCAACAT- CTGCTCCAGAGTCACCGAAG; S286C, GGCAACATCAT- GTGTAGAGTCACCGAAGA; R287C, GCAACATCATGTC- CTGCGTCACCGAAGATAC; V288C, CGGCAACATCAT- GTCCAGATGCACCGAAGATACG; and R659C, CAAGC- CTCTGCCTCAGTACG.
X
ABCB2 p.Gln277Cys 17164240:39:66
status: NEWX
ABCB2 p.Gln277Cys 17164240:39:277
status: NEW40 Expression of Human TAP Mutants-The single cysteine TAP1 mutants, Q277C, G282C, N283C, I284C, M285C, S286C, R287C, V288C, and R659C, were generated by ligase chain reaction with the following primers using cysteine-less human TAP1 with a C-terminal His10 tag as template (16): Q277C, CCGAATTCTTCCAGTGCAACCAGACCGC; G282C, GCA- GAACCAGACCTGCAACATCATGTCC; N283C, CAGAAC- CAGACCGGCTGCATCATGTCCAGAG; I284C, GACCGG- CAACTGCATGTCCAGAG; M285C, GACCGGCAACAT- CTGCTCCAGAGTCACCGAAG; S286C, GGCAACATCAT- GTGTAGAGTCACCGAAGA; R287C, GCAACATCATGTC- CTGCGTCACCGAAGATAC; V288C, CGGCAACATCAT- GTCCAGATGCACCGAAGATACG; and R659C, CAAGC- CTCTGCCTCAGTACG.
X
ABCB2 p.Gln277Cys 17164240:40:66
status: NEWX
ABCB2 p.Gln277Cys 17164240:40:277
status: NEW