ABCMdb

PMID: 17164240

Herget M, Oancea G, Schrodt S, Karas M, Tampe R, Abele R
Mechanism of substrate sensing and signal transmission within an ABC transporter: use of a Trojan horse strategy.
J Biol Chem. 2007 Feb 9;282(6):3871-80. Epub 2006 Dec 12., [PubMed]

Sentences

No. Mutations Sentence Comment

39 ABCB2 p.Gln277Cys ABCB2 p.Gln277Cys ABCB3 p.Met285Cys ABCB3 p.Met285Cys Expression of Human TAP Mutants-The single cysteine TAP1 mutants, Q277C, G282C, N283C, I284C, M285C, S286C, R287C, V288C, and R659C, were generated by ligase chain reaction with the following primers using cysteine-less human TAP1 with a C-terminal His10 tag as template (16): Q277C, CCGAATTCTTCCAGTGCAACCAGACCGC; G282C, GCA- GAACCAGACCTGCAACATCATGTCC; N283C, CAGAAC- CAGACCGGCTGCATCATGTCCAGAG; I284C, GACCGG- CAACTGCATGTCCAGAG; M285C, GACCGGCAACAT- CTGCTCCAGAGTCACCGAAG; S286C, GGCAACATCAT- GTGTAGAGTCACCGAAGA; R287C, GCAACATCATGTC- CTGCGTCACCGAAGATAC; V288C, CGGCAACATCAT- GTCCAGATGCACCGAAGATACG; and R659C, CAAGC- CTCTGCCTCAGTACG. Login to comment 40 ABCB2 p.Gln277Cys ABCB2 p.Gln277Cys ABCB3 p.Met285Cys ABCB3 p.Met285Cys Expression of Human TAP Mutants-The single cysteine TAP1 mutants, Q277C, G282C, N283C, I284C, M285C, S286C, R287C, V288C, and R659C, were generated by ligase chain reaction with the following primers using cysteine-less human TAP1 with a C-terminal His10 tag as template (16): Q277C, CCGAATTCTTCCAGTGCAACCAGACCGC; G282C, GCA- GAACCAGACCTGCAACATCATGTCC; N283C, CAGAAC- CAGACCGGCTGCATCATGTCCAGAG; I284C, GACCGG- CAACTGCATGTCCAGAG; M285C, GACCGGCAACAT- CTGCTCCAGAGTCACCGAAG; S286C, GGCAACATCAT- GTGTAGAGTCACCGAAGA; R287C, GCAACATCATGTC- CTGCGTCACCGAAGATAC; V288C, CGGCAACATCAT- GTCCAGATGCACCGAAGATACG; and R659C, CAAGC- CTCTGCCTCAGTACG. Login to comment