ABCMdb

PMID: 9521779

Urbatsch IL, Beaudet L, Carrier I, Gros P
Mutations in either nucleotide-binding site of P-glycoprotein (Mdr3) prevent vanadate trapping of nucleotide at both sites.
Biochemistry. 1998 Mar 31;37(13):4592-602., 1998-03-31 [PubMed]

Sentences

No. Mutations Sentence Comment

29 ABCB1 p.Lys1076Met ABCB1 p.Lys433Met In purified reconstituted human MDR1, mutations at the corresponding positions (K433M, K1076M) abolished verapamil-stimulated ATPase activity (24). Login to comment 254 ABCC7 p.Lys1250Ala ABCC7 p.Lys464Ala ABCC7 p.Lys1250Met In the cystic fibrosis transmembrane conductance regulator (CFTR), mutations of the conserved Walker A lysine altered the conductive properties of the Cl- channel: the K464A mutation in NB1 decreased the frequency of channel openings, whereas K1250A or K1250M in NB2 prolonged the open state of the channel (59). Login to comment 265 ABCC8 p.Asp854Asn ABCC8 p.Lys719Arg ABCC8 p.Lys719Met Point mutations in the Walker A and B motifs of NB1, K719R, K719M, and D854N impaired 8-azido[R-32 P]ATP binding, whereas NB2 mutations, K1385R, K1385M, and D1506N, retained their ability to bind low concentrations of 8-azido[R-32P]ATP in the presence or absence of Mg2+ (65). Login to comment