ABCMdb

PMID: 9379898

Wemmie JA, Moye-Rowley WS
Mutational analysis of the Saccharomyces cerevisiae ATP-binding cassette transporter protein Ycf1p.
Mol Microbiol. 1997 Aug;25(4):683-94., [PubMed]

Sentences

No. Mutations Sentence Comment

133 ABCC1 p.Gly756Asp ABCC7 p.Gly551Asp ABCC7 p.Gly551Ser ABCC7 p.Arg553Gln ABCC7 p.Lys464Met ABCC7 p.Arg553Met ABCC1 p.Gly756Ser These mutants corresponded to CFTR alterations known to be associated with cystic fibrosis (G551D and G551S in CFTR, G756D and G756S in Ycf1p) as well as lesions that either disturb normal function (K464M in CFTR, K669M in Ycf1p) or act to suppress the phenotype of ⌬F508 CFTR (R553Q and R553M in CFTR, K758Q and K758M in CFTR). Login to comment 135 ABCC1 p.Gly756Asp ABCC1 p.Gly756Ser K669M, G756S and G756D were unable to complement the cadmium hypersensitivity of the ⌬ycf1 strain. Western blot analysis of these mutant Ycf1p derivatives indicated that all these proteins were produced at the same level in the cell. Login to comment 137 ABCC7 p.Lys464Met The analogous mutations in the CFTR are also non-functional but localized correctly, with the exception of the K464M mutant, which does not function normally and is apparently not properly targeted (Gregory et al., 1991). Login to comment 161 ABCC7 p.Arg553Gln ABCC7 p.Arg553Met The two ⌬F508 suppressor mutations isolated in this study were R553Q and R553M. Login to comment 193 ABCC7 p.Arg553Gln ABCC7 p.Arg553Met (Paddon et al., 1996) has shown that the R553Q and R553M mutations do not act by correcting a mislocalization defect in the Ste6p-⌬F508 CFTR chimera. Login to comment 237 ABCC1 p.Gly756Asp ABCC7 p.Gly551Asp ABCC7 p.Gly551Ser ABCC1 p.Gly756Ser The three mutant Ycf1p derivatives (⌬F713, G756D and G756S) that correspond to known CF-causing alleles of CFTR (⌬F508, G551D and G551S) all produce a Ycf1p mutant that exhibits a defect analogous to its CFTR counterpart. Login to comment 238 ABCC7 p.Lys464Met A mutation not associated with CF (K669M in Ycf1p, K464M in CFTR) produced a non-functional, vacuolar membrane-localized protein when present in Ycf1p but was apparently retained in the endoplasmic reticulum in the context of the CFTR (Gregory et al., 1991). Login to comment 281 ABCC1 p.Gly756Asp ABCC1 p.Gly756Ser The 2 ␮m plasmids carrying the indicated mutant forms of YCF1 and the relevant mutagenic PCR primers are listed below: pJAW100 (G756S), 5Ј-ATC TCC TTA TCT GGA TCC CAA AAA GCT CGT TTG-3Ј; pJAW88 (G756D), 5Ј-ATC TCC TTA TCT GGA GAC CAA AAA GCT CGT TTG-3Ј; pJAW86 (K669M), 5Ј- AAA GTT GGC AGT GGT ATG ACA GCT CTA TT-3Ј; pJAW98 (K758R), 5Ј-TTA TCT GGA GGA CAA CGG GCC CGT TTG TCT TTA-3Ј; pJAW108 (K758M), 5Ј-AGA CAA ACG AGC CAT TTG TCC TCC AGA TAA GGA TAT CCC TTT C-3Ј; pJAW109 (K758Q), 5Ј-AGA CAA ACG AGC TTG TTG TCC TCC AGA TAA GCT TAT CCC TTT C-3Ј. Login to comment