ABCMdb

PMID: 9376123

Berguerand M, Klapisz E, Thomas G, Humbert L, Jouniaux AM, Olivier JL, Bereziat G, Masliah J
Differential stimulation of cytosolic phospholipase A2 by bradykinin in human cystic fibrosis cell lines.
Am J Respir Cell Mol Biol. 1997 Oct;17(4):481-90., [PubMed]

Sentences

No. Mutations Sentence Comment

25 ABCC7 p.Asn1303Lys ABCC7 p.Ser549Asn Abbreviations: arachidonic acid, AA; cystic fibrosis, CF; cystic fibrosis transmembrane conductance regulator, CFTR; cystic fibrosis cell line presenting two minor mutations S549N and N1303K, CFT-1; cystic fibrosis cell line presenting ⌬F508 mutation, CFT-2; enhanced chemiluminescence, ECL; (1,4,5)-inositol triphosphate, IP3; normal cell line, NT-1; phospholipase A2, PLA2; phospholipase C, PLC; phenylmethylsulfonyl fluoride, PMSF; tetradecanoyl phorbol acetate, TPA. Login to comment 43 ABCC7 p.Asn1303Lys ABCC7 p.Asn1303Lys ABCC7 p.Ser549Asn ABCC7 p.Ser549Asn We have examined the mechanism by which the ⌬F508 CFTR mutation affects AA release by comparing the coupling of bradykinin receptors to the activation of PLC and PLA2 in a ⌬F508 tracheal epithelial cell line, a double heterozygote cell line (S549N, N1303K), and in a control cell line. Login to comment 67 ABCC7 p.Asn1303Lys ABCC7 p.Ser549Asn Three cell lines were studied: a control cell line (NT-1), a CF cell line carrying the homozygous mutation DF508 of CFTR (CFT-2) and a CF cell line carrying a double heterozygous mutation S549N and N1303K (CFT-1). Login to comment 68 ABCC7 p.Asn1303Lys ABCC7 p.Ser549Asn Three cell lines were studied: a control cell line (NT-1), a CF cell line carrying the homozygous mutation ⌬F508 of CFTR (CFT-2) and a CF cell line carrying a double heterozygous mutation S549N and N1303K (CFT-1). Login to comment 117 ABCC7 p.Asn1303Lys ABCC7 p.Asn1303Lys ABCC7 p.Ser549Asn ABCC7 p.Ser549Asn Results Cytosolic PLA2 Activity Specific for AA in Epithelial Cystic Fibrosis Cell Lines We have shown that an epithelial ⌬F508 cell line (CFT-2) releases more AA in response to bradykinin than do the control epithelial cell line (NT-1), or a CF epithelial cell line bearing the double heterozygous mutation S549N and N1303K (CFT-1). Login to comment 176 ABCC7 p.Asn1303Lys ABCC7 p.Asn1303Lys ABCC7 p.Ser549Asn ABCC7 p.Ser549Asn The maximal concentration in ⌬F508 epithelial cells was 3 times greater than in the control cells or in S549N-N1303K CF cells (Figure 5). Login to comment