ABCMdb

PMID: 21203549

Taghizadeh R, Noh M, Huh YH, Ciusani E, Sigalotti L, Maio M, Arosio B, Nicotra MR, Natali P, Sherley JL, La Porta CA
CXCR6, a newly defined biomarker of tissue-specific stem cell asymmetric self-renewal, identifies more aggressive human melanoma cancer stem cells.
PLoS One. 2010 Dec 22;5(12):e15183., [PubMed]

Sentences

No. Mutations Sentence Comment

80 ABCG2 p.Phe208Ser ABCG2 p.Ser248Thr First, as shown in Table 1, we analyzed three polymorphisms C/A (421 C.A), C/T (S248T) and C/T (F208S) in the unsorted populations and in the ABCG2+ and ABCG2- sorted subpopulations revealing a heterozygous status of ABCG2 for all the three polymorphisms. Login to comment 83 ABCG2 p.Phe208Ser ABCG2 p.Phe208Ser ABCG2 p.Ser248Pro Moreover, F208S and S248P polymorphisms were associated with defective active transport of methotrexate and haematoporphyrin [24]; and, in particular, F208S variant proteins (both-glycosylated and immature forms) are recognized as misfolded proteins by putative ''check point`` systems and readily undergo ubiquination and protein degradation in proteasomes [25]. Login to comment 103 ABCG2 p.Phe208Ser ABCG2 p.Ser248Pro Cells 421 C.A (C/A) S248P (C/T) F208S (C/T) IGR37 CA CT CT IGR39 CA CT CT ABCG2+ IGR37 CA CT CT ABCG2- IGR37 CA CT CT ABCG2+ IGR39 CA CT CT ABCG2- IGR39 CA CT CT doi:10.1371/journal.pone.0015183.t001 unsorted IGR37 cells (0.13 grams vs. 1.4 grams, respectively; p,0.01) 2 months after the injection of the cells (Table 1). Login to comment 297 ABCG2 p.Phe208Ser ABCG2 p.Ser248Pro SNP analysis DNA was extracted from cells using a salting-out method [28] Genotyping for ABCG2 421 C.A (assay ID: C__15854163_70), S248P (assay ID: C__27458615_40) and F208S (assay ID: C__8826940_10) single nucleotide polymorphisms (SNPs) were performed using Validated TaqMan Genotyping Assay (Applied Biosystems). Login to comment