ABCMdb

PMID: 16621508

Mannelli I, Lecerf L, Guerrouache M, Goossens M, Millot MC, Canva M
DNA immobilisation procedures for surface plasmon resonance imaging (SPRI) based microarray systems.
Biosens Bioelectron. 2007 Jan 15;22(6):803-9. Epub 2006 Apr 18., [PubMed]

Sentences

No. Mutations Sentence Comment

8 ABCC7 p.Gln493* ABCC7 p.Val520Phe Two deletions of three bases (F508 and I507) and four single nucleotide polymorphisms (M470V, Q493X, V520F and 1716 G > A) were investigated. Login to comment 56 ABCC7 p.Gln493* ABCC7 p.Gln493* ABCC7 p.Gln493* ABCC7 p.Gln493* ABCC7 p.Val520Phe ABCC7 p.Val520Phe ABCC7 p.Val520Phe ABCC7 p.Val520Phe The probe (P) sequences used are: 1, F508 wild type (P-F508-WT) = 5 biotin (T)17 ATA TCA TCT TTG GTG 3; 2, F508 mutant (P-F508-M) = 5 biotin (T)17 AAT ATC ATT GGT GTT 3; 3, I507 wild type (P-I507-WT) = 5 biotin (T)15 GAA AAT ATC ATC TTT G 3; 4, I507 mutant (P-I507-M) = 5 biotin (T)15 GAA AAT ATC TTT GGT 3; 5, M470V wild type (P-M470V-WT) = 5 biotin (T)16 TTC TAA TGA TGA TTA 3; 7, M470V mutant (P-M470V-M) = 5 biotin (T)16 TTC TAA TGG TGA TTA 3; 9, 1716 G > A wild type (P-1716 G > A-WT) = 5 biotin (T)18 AGA AGA GGT AAG A 3; 11, 1716 G > A mutant (P-1716 G > A-M) = 5 biotin (T)18 AGA AGA AGT AAG A 3; 13, Q493X wild type (P-Q493X-WT) = 5 biotin (T)18 TGT TCT CAG TTT T 3; 15, Q493X mutant (P-Q493X-M) = 5 biotin (T)18 TGT TCT TAG TTT T 3; 17, V520F wild type (P-V520F-WT) = 5 biotin (T)19 GAA GCG TCA TC 3; 19, V520F mutant (P-V520F-M) = 5 biotin (T)19 GAA GCT TCA TC 3 and 21, not relevant (P-NR) = 5 biotin (T)18 CAC TTC GTG CCT T 3. Login to comment 61 ABCC7 p.Gln493* ABCC7 p.Gln493* ABCC7 p.Val520Phe ABCC7 p.Val520Phe In the case of the remaining polymorphisms, the correspondence with the official nomenclature is: M470V-WT = c.1540A (normal allele), M470V-M = c.1540G (mutant allele), Q493X- WT = c.1609C (normal allele), Q493X-N = c.1609T (mutant allele), 1716 G > A-WT = c.1716G (normal allele), 1716 G > AM = c.1716A (mutant allele), V520F-WT = c.1690G (normal allele) and V520F-M = c.1690T (mutant allele). Login to comment