ABCMdb

PMID: 15463939

Sheppard DN, Gray MA, Gong X, Sohma Y, Kogan I, Benos DJ, Scott-Ward TS, Chen JH, Li H, Cai Z, Gupta J, Li C, Ramjeesingh M, Berdiev BK, Ismailov II, Bear CE, Hwang TC, Linsdell P, Hug MJ
The patch-clamp and planar lipid bilayer techniques: powerful and versatile tools to investigate the CFTR Cl- channel.
J Cyst Fibros. 2004 Aug;3 Suppl 2:101-8., [PubMed]

Sentences

No. Mutations Sentence Comment

142 ABCC7 p.Arg347Asp Importantly, as with all mutagenesis studies, there are important caveats: mutations within the CFTR pore might cause indirect and potentially global changes in pore architecture (e.g., R347D, [20]). Login to comment 143 ABCC7 p.Arg352Gln Alternatively, mutations might disrupt channel gating (e.g., R352Q, [21]). Login to comment 146 ABCC7 p.Arg347Asp Importantly, as with all mutagenesis studies, there are important caveats: mutations within the CFTR pore might cause indirect and potentially global changes in pore architecture (e.g., R347D, [20]). Login to comment 147 ABCC7 p.Arg352Gln Alternatively, mutations might disrupt channel gating (e.g., R352Q, [21]). Login to comment 148 ABCC7 p.Lys1250Ala To investigate the gating kinetics of the brief flickery closures interrupting channel openings, Zhou et al. [22] used K1250A, a CFTR construct with an elevated Po ( Po f 0.9), and a simple model of channel block: Open X a b Blocked; &#f0;6&#de; where a is the apparent on-rate of the blocker and b the off-rate. Login to comment 153 ABCC7 p.Lys1250Ala To investigate the gating kinetics of the brief flickery closures interrupting channel openings, Zhou et al. [22] used K1250A, a CFTR construct with an elevated Po ( Po f 0.9), and a simple model of channel block: Open X a b Blocked; ð6Þ where a is the apparent on-rate of the blocker and b the off-rate. Login to comment