ABCA1 p.Tyr1206Phe
| Predicted by SNAP2: | A: D (80%), C: D (80%), D: D (91%), E: D (91%), F: N (61%), G: D (85%), H: D (91%), I: D (59%), K: D (95%), L: D (59%), M: D (80%), N: D (91%), P: D (95%), Q: D (91%), R: D (91%), S: D (91%), T: D (91%), V: D (75%), W: D (91%), |
| Predicted by PROVEAN: | A: D, C: D, D: D, E: D, F: N, G: D, H: D, I: D, K: D, L: D, M: D, N: D, P: D, Q: D, R: D, S: D, T: D, V: D, W: D, |
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[hide] Insulin down-regulates specific activity of ATP-bi... Atherosclerosis. 2011 Jun;216(2):334-41. Epub 2011 Feb 22. Nonomura K, Arai Y, Mitani H, Abe-Dohmae S, Yokoyama S
Insulin down-regulates specific activity of ATP-binding cassette transporter A1 for high density lipoprotein biogenesis through its specific phosphorylation.
Atherosclerosis. 2011 Jun;216(2):334-41. Epub 2011 Feb 22., [PMID:21402379]
Abstract [show]
Insulin resistance/hyperinsulinism is one of the major risks for atherosclerotic vascular diseases and low HDL may be involved in pathogenesis. We examined direct effects of insulin on HDL biosynthesis focusing on the activity of ATP-binding cassette transporter A1 (ABCA1) in culture cells and in experimental animals. Insulin impairs HDL biosynthesis through modulation of ABCA1 activity by two different mechanisms. Insulin enhances degradation of ABCA1. However, even after this effect was cancelled by blocking its specific signal, insulin still reduces HDL biogenesis. This effect was found due to phosphorylation of ABCA1 that leads to decrease of its specific activity. We identified a novel insulin-specific phosphorylation site Tyr1206 of ABCA1 to regulate its specific activity. The observation in a rat model of insulin resistance was consistent with these results. The findings demonstrate a new mechanism for regulation of ABCA1 activity and provide new insights into the link between development of atherosclerosis, and insulin resistance/hyperinsulinism.
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No. Sentence Comment
151 Wild type and Y1206F ABCA1 genes were transfected into HEK293 cells.
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ABCA1 p.Tyr1206Phe 21402379:151:14
status: NEW154 Wild type, Y1206F and Y1206E were transfected to HEK293 cells (E).
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ABCA1 p.Tyr1206Phe 21402379:154:11
status: NEW171 In order to identify the residue(s) for function-related phosphorylation, four ABCA1 mutant genes were constructed, in which each tyrosine residue was substituted to phenylalanine (Y958F, Y1206F, Y2041F, and Y2045F).
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ABCA1 p.Tyr1206Phe 21402379:171:188
status: NEW174 The inhibitory effect of insulin on the lipid release completely diminished in the Y1206F mutant cells, while the effect remained with all other mutants (Fig. 4C).
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ABCA1 p.Tyr1206Phe 21402379:174:83
status: NEW175 Phosphorylation of ABCA1 by insulin disappeared in substitution mutant of Y1206F while phosphorylation of IR was uninfluenced (Fig. 4D).
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ABCA1 p.Tyr1206Phe 21402379:175:74
status: NEW177 The expression levels of the Y1206 mutants (Y1206F and Y1206E) were comparable to that of wild type ABCA1 (Fig. 4E).
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ABCA1 p.Tyr1206Phe 21402379:177:44
status: NEW