ABCMdb

PMID: 21402379

Nonomura K, Arai Y, Mitani H, Abe-Dohmae S, Yokoyama S
Insulin down-regulates specific activity of ATP-binding cassette transporter A1 for high density lipoprotein biogenesis through its specific phosphorylation.
Atherosclerosis. 2011 Jun;216(2):334-41. Epub 2011 Feb 22., [PubMed]

Sentences

No. Mutations Sentence Comment

151 ABCA1 p.Tyr1206Phe Wild type and Y1206F ABCA1 genes were transfected into HEK293 cells. Login to comment 154 ABCA1 p.Tyr1206Phe ABCA1 p.Tyr1206Glu Wild type, Y1206F and Y1206E were transfected to HEK293 cells (E). Login to comment 171 ABCA1 p.Tyr1206Phe ABCA1 p.Tyr958Phe ABCA1 p.Tyr2045Phe ABCA1 p.Tyr2041Phe In order to identify the residue(s) for function-related phosphorylation, four ABCA1 mutant genes were constructed, in which each tyrosine residue was substituted to phenylalanine (Y958F, Y1206F, Y2041F, and Y2045F). Login to comment 174 ABCA1 p.Tyr1206Phe The inhibitory effect of insulin on the lipid release completely diminished in the Y1206F mutant cells, while the effect remained with all other mutants (Fig. 4C). Login to comment 175 ABCA1 p.Tyr1206Phe Phosphorylation of ABCA1 by insulin disappeared in substitution mutant of Y1206F while phosphorylation of IR was uninfluenced (Fig. 4D). Login to comment 176 ABCA1 p.Tyr1206Glu For further verification of the specific phosphorylation at this residue by insulin, a negative charge was introduced to 1206 substituting tyrosine by glutamine, mimicking a phosphorylated form (Y1206E). Login to comment 177 ABCA1 p.Tyr1206Phe ABCA1 p.Tyr1206Glu The expression levels of the Y1206 mutants (Y1206F and Y1206E) were comparable to that of wild type ABCA1 (Fig. 4E). Login to comment 178 ABCA1 p.Tyr1206Glu Lipid release by apoA-I was completely suppressed in the cells transiently expressing Y1206E mutant (Fig. 4F). Login to comment