ABCA1 p.Tyr1206Phe

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PMID: 21402379 [PubMed] Nonomura K et al: "Insulin down-regulates specific activity of ATP-binding cassette transporter A1 for high density lipoprotein biogenesis through its specific phosphorylation."
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151 Wild type and Y1206F ABCA1 genes were transfected into HEK293 cells.
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ABCA1 p.Tyr1206Phe 21402379:151:14
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154 Wild type, Y1206F and Y1206E were transfected to HEK293 cells (E).
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ABCA1 p.Tyr1206Phe 21402379:154:11
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171 In order to identify the residue(s) for function-related phosphorylation, four ABCA1 mutant genes were constructed, in which each tyrosine residue was substituted to phenylalanine (Y958F, Y1206F, Y2041F, and Y2045F).
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ABCA1 p.Tyr1206Phe 21402379:171:188
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174 The inhibitory effect of insulin on the lipid release completely diminished in the Y1206F mutant cells, while the effect remained with all other mutants (Fig. 4C).
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ABCA1 p.Tyr1206Phe 21402379:174:83
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175 Phosphorylation of ABCA1 by insulin disappeared in substitution mutant of Y1206F while phosphorylation of IR was uninfluenced (Fig. 4D).
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ABCA1 p.Tyr1206Phe 21402379:175:74
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177 The expression levels of the Y1206 mutants (Y1206F and Y1206E) were comparable to that of wild type ABCA1 (Fig. 4E).
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ABCA1 p.Tyr1206Phe 21402379:177:44
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