ABCD1 p.Glu458Ala
| Predicted by SNAP2: | A: N (87%), C: N (93%), D: N (93%), F: N (82%), G: N (82%), H: N (93%), I: N (87%), K: N (87%), L: N (87%), M: N (82%), N: N (93%), P: N (78%), Q: N (93%), R: N (82%), S: N (93%), T: N (93%), V: N (87%), W: N (53%), Y: N (66%), |
| Predicted by PROVEAN: | A: N, C: N, D: N, F: N, G: N, H: N, I: N, K: N, L: N, M: N, N: N, P: N, Q: N, R: N, S: N, T: N, V: N, W: D, Y: N, |
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[hide] Identification of in vivo substrates of the chaper... Biosci Biotechnol Biochem. 2007 Apr;71(4):1073-7. Epub 2007 Apr 7. Endo A, Kurusu Y
Identification of in vivo substrates of the chaperonin GroEL from Bacillus subtilis.
Biosci Biotechnol Biochem. 2007 Apr;71(4):1073-7. Epub 2007 Apr 7., [PMID:17420574]
Abstract [show]
We investigated GroEL substrates from Bacillus subtilis 168 using the single-ring mutant of B. subtilis GroEL. We identified 28 candidates for GroEL substrates, of which Spo0B, Ald, Eno, SpoIIP, and FbaA were involved in spore formation, and Rnc, Tuf, Eno, Tsf, and FbaA were essential for B. subtilis growth. As observed at the protein level, the amount of SpoIIP interaction with GroEL increased at 3 h after initiation of sporulation.
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No. Sentence Comment
14 Mutations (R449E, E458A, S460A, and V461A in B. subtilis) were introduced by oligonucleotide-directed mutagenesis of the pBL30 vector, in accordance with the method of Weissman et al.7) Using the resulting construct (pBL-SRI30) as a template, the Shine-Dalgarno sequence plus the His6-groEL SRI fusion gene were amplified by PCR using NHisSRI-F (50 -GTGACTAGTTAACAATTTCACAC-30 ; SpeI site underlined) and H30BL-R primers (see above).
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ABCD1 p.Glu458Ala 17420574:14:18
status: NEW