PMID: 17420574

Endo A, Kurusu Y
Identification of in vivo substrates of the chaperonin GroEL from Bacillus subtilis.
Biosci Biotechnol Biochem. 2007 Apr;71(4):1073-7. Epub 2007 Apr 7., [PubMed]
Sentences
No. Mutations Sentence Comment
11 ABCD1 p.Arg452Glu
X
ABCD1 p.Arg452Glu 17420574:11:47
status: NEW
view ABCD1 p.Arg452Glu details
 This mutant has four amino acid substitutions (R452E, E461A, S463A, and V464A, in E. coli GroEL) that prevent the formation of a double-ring structure and the release of GroES,7) and hence substrates can be observed to accumulate in the closed folding cage formed by the molecule. Login to comment 14 ABCD1 p.Glu458Ala
X
ABCD1 p.Glu458Ala 17420574:14:18
status: NEW
view ABCD1 p.Glu458Ala details
 Mutations (R449E, E458A, S460A, and V461A in B. subtilis) were introduced by oligonucleotide-directed mutagenesis of the pBL30 vector, in accordance with the method of Weissman et al.7) Using the resulting construct (pBL-SRI30) as a template, the Shine-Dalgarno sequence plus the His6-groEL SRI fusion gene were amplified by PCR using NHisSRI-F (50 -GTGACTAGTTAACAATTTCACAC-30 ; SpeI site underlined) and H30BL-R primers (see above). Login to comment