ABCD1 p.Glu458Ala
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PMID: 17420574
[PubMed]
Endo A et al: "Identification of in vivo substrates of the chaperonin GroEL from Bacillus subtilis."
No.
Sentence
Comment
14
Mutations (R449E, E458A, S460A, and V461A in B. subtilis) were introduced by oligonucleotide-directed mutagenesis of the pBL30 vector, in accordance with the method of Weissman et al.7) Using the resulting construct (pBL-SRI30) as a template, the Shine-Dalgarno sequence plus the His6-groEL SRI fusion gene were amplified by PCR using NHisSRI-F (50 -GTGACTAGTTAACAATTTCACAC-30 ; SpeI site underlined) and H30BL-R primers (see above).
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ABCD1 p.Glu458Ala 17420574:14:18
status: NEW