ABCC8 p.Leu1210Arg
| Predicted by SNAP2: | A: N (53%), C: N (66%), D: D (85%), E: D (80%), F: D (59%), G: D (75%), H: D (59%), I: N (87%), K: D (80%), M: N (66%), N: D (80%), P: D (85%), Q: D (63%), R: D (80%), S: D (63%), T: N (66%), V: N (93%), W: D (75%), Y: D (66%), |
| Predicted by PROVEAN: | A: D, C: D, D: D, E: D, F: D, G: D, H: D, I: N, K: D, M: N, N: D, P: D, Q: D, R: D, S: D, T: D, V: D, W: D, Y: D, |
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[hide] Interferon removes its own receptors as it blocks ... Eur J Biochem. 1988 Feb 1;171(3):675-82. Eid P, Bandu MT, Uze G, Mogensen KE
Interferon removes its own receptors as it blocks the division of Daudi cells.
Eur J Biochem. 1988 Feb 1;171(3):675-82., [PMID:2964367]
Abstract [show]
The Burkitt-derived line, Daudi, whose proliferation is inhibited by human alpha-interferon (IFN-alpha), was treated with 125I-labelled recombinant human IFN-alpha A. After separation from unbound ligand, cell-bound IFN was extracted with the detergent digitonin yielding soluble and insoluble complexes of IFN and receptor, together with a certain amount of uncomplexed IFN. 1. Soluble complexes were stable enough to be separated from uncomplexed IFN by permeation chromatography. Treatment of soluble complexes with the bifunctional reagent, disuccinimidyl suberate, yielded a radioactive product separating with an Mr of 130,000 on electrophoresis in sodium dodecyl sulphate. Similar complexes could be recovered with sodium dodecyl sulphate from the digitonin-insoluble residue, treated with the bi-functional reagent. 2. The total (soluble and insoluble) of complexed IFN obtained after digitonin extraction was a constant fraction (0.62) of the total cell-bound radioactivity, being independent of the concentration of IFN added to the cells (less than pM to greater than nM), and of the time of incubation (1 min to 20 h). However, between 30 min and 3 h of incubation, the insoluble complex increased, at the expense of the soluble complex, and there appeared a cellular pool of degraded ligand. From 3 h to 20 h the distribution of ligand-derived radioactivity remained constant while the total amount decreased to less than 10% of its value at 30 min. This decrease in binding was matched by the appearance of an equivalent quantity of radiolabelled fragments in the culture medium. 3. The inhibition of cellular division due to IFN was shown to be coincident with the disappearance of cellular binding and with the cell-mediated degradation of receptor-complexed IFN. We propose that IFN removes its own receptor and, in doing so, blocks a linked function necessary for the stimulated growth of Daudi cells.
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No. Sentence Comment
86 (A) The neutral cxtract from Daudi cells treated with IFN at 75 pM for 30 min; (B) as in A but with a monoclonal antibody to IFN present; (C)profile for IFN and antibody alone; (D) same as A but with the digitonin at pH 3 (0.1 M acetic acid); (E)same as A but with 1 mM 1,4-dithioerythritolpresent; (F) extract from untreated Daudi cells to which IFN and 1,4-dithioerythritol had been added together; (G) extract from untreated Daudi cells to which IFN has been added alone; (H) extract from untreated mouse cells (L1210R) to which IFN has been added alone approximately linear on log M , of the protein markers.
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ABCC8 p.Leu1210Arg 2964367:86:515
status: NEW116 Fig. 2G (Daudi extract) and Fig. 2H (mouse L1210R extract) suggest that scrambling is probably unrelated to receptor binding as the R strain of the mouse lymphoma line L1210 is resistant to IFN and possesses no receptors [20].
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ABCC8 p.Leu1210Arg 2964367:116:43
status: NEW