ABCB1 p.Glu556Gln

Predicted by SNAP2: A: D (85%), C: D (80%), D: N (61%), F: D (91%), G: D (91%), H: D (85%), I: D (85%), K: D (91%), L: D (91%), M: N (72%), N: D (85%), P: D (91%), Q: N (53%), R: D (91%), S: D (85%), T: D (85%), V: D (85%), W: D (91%), Y: D (91%),
Predicted by PROVEAN: A: D, C: D, D: D, F: D, G: D, H: D, I: D, K: D, L: D, M: D, N: D, P: D, Q: D, R: D, S: D, T: D, V: D, W: D, Y: D,

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[hide] Sauna ZE, Muller M, Peng XH, Ambudkar SV
Importance of the conserved Walker B glutamate residues, 556 and 1201, for the completion of the catalytic cycle of ATP hydrolysis by human P-glycoprotein (ABCB1).
Biochemistry. 2002 Nov 26;41(47):13989-4000., 2002-11-26 [PMID:12437356]

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[hide] Ambudkar SV, Kimchi-Sarfaty C, Sauna ZE, Gottesman MM
P-glycoprotein: from genomics to mechanism.
Oncogene. 2003 Oct 20;22(47):7468-85., 2003-10-20 [PMID:14576852]

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[hide] Carrier I, Julien M, Gros P
Analysis of catalytic carboxylate mutants E552Q and E1197Q suggests asymmetric ATP hydrolysis by the two nucleotide-binding domains of P-glycoprotein.
Biochemistry. 2003 Nov 11;42(44):12875-85., 2003-11-11 [PMID:14596601]

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[hide] Tombline G, Bartholomew LA, Urbatsch IL, Senior AE
Combined mutation of catalytic glutamate residues in the two nucleotide binding domains of P-glycoprotein generates a conformation that binds ATP and ADP tightly.
J Biol Chem. 2004 Jul 23;279(30):31212-20. Epub 2004 May 24., 2004-07-23 [PMID:15159388]

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[hide] Sauna ZE, Nandigama K, Ambudkar SV
Exploiting reaction intermediates of the ATPase reaction to elucidate the mechanism of transport by P-glycoprotein (ABCB1).
J Biol Chem. 2006 Sep 8;281(36):26501-11. Epub 2006 Jul 14., 2006-09-08 [PMID:16844693]

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[hide] Sauna ZE, Ambudkar SV
About a switch: how P-glycoprotein (ABCB1) harnesses the energy of ATP binding and hydrolysis to do mechanical work.
Mol Cancer Ther. 2007 Jan;6(1):13-23., [PMID:17237262]

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[hide] Zolnerciks JK, Wooding C, Linton KJ
Evidence for a Sav1866-like architecture for the human multidrug transporter P-glycoprotein.
FASEB J. 2007 Dec;21(14):3937-48. Epub 2007 Jul 12., [PMID:17627029]

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[hide] Loo TW, Bartlett MC, Clarke DM
Nucleotide binding, ATP hydrolysis, and mutation of the catalytic carboxylates of human P-glycoprotein cause distinct conformational changes in the transmembrane segments.
Biochemistry. 2007 Aug 14;46(32):9328-36. Epub 2007 Jul 18., 2007-08-14 [PMID:17636884]

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[hide] Sauna ZE, Kim IW, Nandigama K, Kopp S, Chiba P, Ambudkar SV
Catalytic cycle of ATP hydrolysis by P-glycoprotein: evidence for formation of the E.S reaction intermediate with ATP-gamma-S, a nonhydrolyzable analogue of ATP.
Biochemistry. 2007 Dec 4;46(48):13787-99. Epub 2007 Nov 8., 2007-12-04 [PMID:17988154]

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[hide] Sauna ZE, Kim IW, Ambudkar SV
Genomics and the mechanism of P-glycoprotein (ABCB1).
J Bioenerg Biomembr. 2007 Dec;39(5-6):481-7., [PMID:18058211]

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[hide] Shukla S, Schwartz C, Kapoor K, Kouanda A, Ambudkar SV
Use of baculovirus BacMam vectors for expression of ABC drug transporters in mammalian cells.
Drug Metab Dispos. 2012 Feb;40(2):304-12. Epub 2011 Oct 31., [PMID:22041108]

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[hide] Loo TW, Bartlett MC, Detty MR, Clarke DM
The ATPase activity of the P-glycoprotein drug pump is highly activated when the N-terminal and central regions of the nucleotide-binding domains are linked closely together.
J Biol Chem. 2012 Aug 3;287(32):26806-16. doi: 10.1074/jbc.M112.376202. Epub 2012 Jun 14., [PMID:22700974]

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[hide] Chufan EE, Kapoor K, Sim HM, Singh S, Talele TT, Durell SR, Ambudkar SV
Multiple transport-active binding sites are available for a single substrate on human P-glycoprotein (ABCB1).
PLoS One. 2013 Dec 5;8(12):e82463. doi: 10.1371/journal.pone.0082463. eCollection 2013., [PMID:24349290]

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[hide] Zolnerciks JK, Akkaya BG, Snippe M, Chiba P, Seelig A, Linton KJ
The Q loops of the human multidrug resistance transporter ABCB1 are necessary to couple drug binding to the ATP catalytic cycle.
FASEB J. 2014 Oct;28(10):4335-46. doi: 10.1096/fj.13-245639. Epub 2014 Jul 11., [PMID:25016028]

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[hide] Loo TW, Clarke DM
Cysteines introduced into extracellular loops 1 and 4 of human P-glycoprotein that are close only in the open conformation spontaneously form a disulfide bond that inhibits drug efflux and ATPase activity.
J Biol Chem. 2014 Sep 5;289(36):24749-58. doi: 10.1074/jbc.M114.583021. Epub 2014 Jul 22., [PMID:25053414]

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[hide] Souza PS, Madigan JP, Gillet JP, Kapoor K, Ambudkar SV, Maia RC, Gottesman MM, Leung Fung K
Expression of the multidrug transporter P-glycoprotein is inversely related to that of apoptosis-associated endogenous TRAIL.
Exp Cell Res. 2015 Aug 15;336(2):318-28. doi: 10.1016/j.yexcr.2015.06.005. Epub 2015 Jun 19., [PMID:26101157]

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