PMID: 22041108

Shukla S, Schwartz C, Kapoor K, Kouanda A, Ambudkar SV
Use of baculovirus BacMam vectors for expression of ABC drug transporters in mammalian cells.
Drug Metab Dispos. 2012 Feb;40(2):304-12. Epub 2011 Oct 31., [PubMed]
Sentences
No. Mutations Sentence Comment
24 ABCB1 p.Glu556Gln
X
ABCB1 p.Glu556Gln 22041108:24:71
status: NEW
view ABCB1 p.Glu556Gln details
 ABBREVIATIONS: ABC, ATP-binding cassette; Pgp, P-glycoprotein; Pgp-EQ, E556Q/E5561206Q nonfunctional Pgp mutant; BacMam, baculovirus-based expression in mammalian cells; FTC, fumitremorgin C; IAAP, iodoarylazidoprazosin; DMEM, Dulbecco`s modified Eagle`s medium; PBS, phosphate-buffered saline; FITC, fluorescein isothiocyanate; ATPase, adenosine triphosphatase; VBL, vinblastine; HEK, human embryonic kidney. Login to comment 75 ABCB1 p.Glu556Gln
X
ABCB1 p.Glu556Gln 22041108:75:51
status: NEW
view ABCB1 p.Glu556Gln details
 In brief, BacMam-Pgp (wild-type) or BacMam-Pgp-EQ (E556Q/E1206Q Pgp mutant that is defective in ATP hydrolysis)-transduced cells (2.5 ϫ 105 cells/well) were grown in a monolayer in a 24-well tissue culture plate at 37°C. Login to comment 110 ABCB1 p.Glu1201Gln
X
ABCB1 p.Glu1201Gln 22041108:110:120
status: NEW
view ABCB1 p.Glu1201Gln details
ABCB1 p.Glu556Gln
X
ABCB1 p.Glu556Gln 22041108:110:113
status: NEW
view ABCB1 p.Glu556Gln details
 In addition, we also transduced HeLa cells with BacMam-Pgp-EQ virus, which expresses a nonfunctional mutant Pgp (E556Q/ E1201Q) with the glutamate residue in the Walker B motif of each nucleotide-binding domain that is changed to glutamine (Sauna et al., 2002). Login to comment