ABCMdb

ABCC1 p.Val20Cys

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Publications

PMID: 9875455 [PubMed] Kim DK et al: "Isolation of a multidrug resistance inhibitor from Aconitum pseudo-laeve var. erectum."

No. Sentence Comment

28 VCR- resistant KB V20C cells (generous gift of Dr. Cheng at Yale University, School of Medicine) were developed from the parental KB cells by stepwise selection for resistance with increasing concentration of VCR and cultured in the presence of 20 nM concentration of VCR. Login to comment
31 Cytotoxicity assay The KB V20C cells were maintained in drug free media 3 days before determination of the concentration capable of inhibiting 50% growth. Login to comment
37 And each fraction was examined for the anti-MDR activity in vitro, and the n-BuOH soluble fraction was found to have anti-MDR activity against KB V20C cells. Login to comment
43 erectum to cancer cells1~ 1 mg/ml IO0 llg/ml I0 llg/ml KB KB KB KB V20C KB7D KB V20C KB7D KB V20C KB7D 1 41.1 16.4 28.6 99.5 56.6 89.9 96.2 95.3 94.2 II 8.3 22.0 9.6 94.2 50.2 85.5 95.3 92 97.4 III 22.7 26.1 6.6 96.9 88.9 93.8 97.4 106.8 100.1 IV 42.5 27.3 6.1 98.5 105.6 95.1 97.9 100.3 98.5 l~Cell viability representsthe concentration IJ.Mof a compound required for 50% inhibition of cell growth. Login to comment
51 The MeOH extract of the roots of Aconitum pseudo-laeve var. erectum NAKAI (Ranunculaceae) showed significant modulating activity of Pgp-MDR against KB V20C but not MRP-MDR. Login to comment

PMID: 9719474 [PubMed] Gaj CL et al: "Decreased drug accumulation without increased drug efflux in a novel MRP-overexpressing multidrug-resistant cell line."

No. Sentence Comment

39 P-gp-expressing KB/V20C [4] cells and KB/MDR cells [25] also have been described previously. Login to comment
41 Drug-resistant cells were maintained in drug-containing medium (7 ␮M of VP-16 for KB/7D cells, 20 nM of vincristine for KB/V20C cells, and 37 nM of doxorubicin for KB/MDR cells). Login to comment
124 A partial revertant cell line, KB/7Dpr , which showed approximately 40-fold resistance to VP-16 and 4-fold resistance to vincristine when compared with KB cells, and known P-gp-expressing cell lines, V20C and KB/MDR, were included in these studies. Login to comment
126 V20C cells also showed no significant MRP expression at the RNA level. Login to comment
129 The KB/7Dpr cells lost most of this overexpression, while KB/7Dr and V20C cells did not show any amplification of the MRP gene. Login to comment
172 In all blots, 1 ‫؍‬ KB, 2 ‫؍Ȃc; KB/7D, 3 ‫؍‬ KB/7Dr , 4 ‫؍‬ KB/7Dpr , 5 ‫؍‬ KB/V20C, and 6 ‫؍‬ KB/MDR cells. Login to comment
192 KB/7D and V20C cells accumulated fewer PLDBs FIG. 2. Login to comment
37 P-gp-expressing KB/V20C [4] cells and KB/MDR cells [25] also have been described previously. Login to comment
122 A partial revertant cell line, KB/7Dpr , which showed approximately 40-fold resistance to VP-16 and 4-fold resistance to vincristine when compared with KB cells, and known P-gp-expressing cell lines, V20C and KB/MDR, were included in these studies. Login to comment
127 The KB/7Dpr cells lost most of this overexpression, while KB/7Dr and V20C cells did not show any amplification of the MRP gene. Login to comment