ABCMdb

PMID: 9719474

Gaj CL, Anyanwutaku I, Chang YH, Cheng YC
Decreased drug accumulation without increased drug efflux in a novel MRP-overexpressing multidrug-resistant cell line.
Biochem Pharmacol. 1998 Apr 15;55(8):1199-211., [PubMed]

Sentences

No. Mutations Sentence Comment

37 ABCC1 p.Val20Cys P-gp-expressing KB/V20C [4] cells and KB/MDR cells [25] also have been described previously. Login to comment 39 ABCC1 p.Val20Cys ABCC1 p.Val20Cys P-gp-expressing KB/V20C [4] cells and KB/MDR cells [25] also have been described previously. Login to comment 41 ABCC1 p.Val20Cys Drug-resistant cells were maintained in drug-containing medium (7 ␮M of VP-16 for KB/7D cells, 20 nM of vincristine for KB/V20C cells, and 37 nM of doxorubicin for KB/MDR cells). Login to comment 122 ABCC1 p.Val20Cys A partial revertant cell line, KB/7Dpr , which showed approximately 40-fold resistance to VP-16 and 4-fold resistance to vincristine when compared with KB cells, and known P-gp-expressing cell lines, V20C and KB/MDR, were included in these studies. Login to comment 124 ABCC1 p.Val20Cys ABCC1 p.Val20Cys A partial revertant cell line, KB/7Dpr , which showed approximately 40-fold resistance to VP-16 and 4-fold resistance to vincristine when compared with KB cells, and known P-gp-expressing cell lines, V20C and KB/MDR, were included in these studies. Login to comment 126 ABCC1 p.Val20Cys V20C cells also showed no significant MRP expression at the RNA level. Login to comment 127 ABCC1 p.Val20Cys The KB/7Dpr cells lost most of this overexpression, while KB/7Dr and V20C cells did not show any amplification of the MRP gene. Login to comment 129 ABCC1 p.Val20Cys The KB/7Dpr cells lost most of this overexpression, while KB/7Dr and V20C cells did not show any amplification of the MRP gene. Login to comment 172 ABCC1 p.Val20Cys ABCC1 p.Val20Cys In all blots, 1 ‫؍‬ KB, 2 ‫؍Ȃc; KB/7D, 3 ‫؍‬ KB/7Dr , 4 ‫؍‬ KB/7Dpr , 5 ‫؍‬ KB/V20C, and 6 ‫؍‬ KB/MDR cells. Login to comment 192 ABCC1 p.Val20Cys KB/7D and V20C cells accumulated fewer PLDBs FIG. 2. Login to comment