ABCMdb

PMID: 9875455

Kim DK, Kwon HY, Lee KR, Rhee DK, Zee OP
Isolation of a multidrug resistance inhibitor from Aconitum pseudo-laeve var. erectum.
Arch Pharm Res. 1998 Jun;21(3):344-7., [PubMed]

Sentences

No. Mutations Sentence Comment

28 ABCC1 p.Val20Cys VCR- resistant KB V20C cells (generous gift of Dr. Cheng at Yale University, School of Medicine) were developed from the parental KB cells by stepwise selection for resistance with increasing concentration of VCR and cultured in the presence of 20 nM concentration of VCR. Login to comment 31 ABCC1 p.Val20Cys Cytotoxicity assay The KB V20C cells were maintained in drug free media 3 days before determination of the concentration capable of inhibiting 50% growth. Login to comment 37 ABCC1 p.Val20Cys And each fraction was examined for the anti-MDR activity in vitro, and the n-BuOH soluble fraction was found to have anti-MDR activity against KB V20C cells. Login to comment 43 ABCC1 p.Val20Cys ABCC1 p.Val20Cys ABCC1 p.Val20Cys erectum to cancer cells1~ 1 mg/ml IO0 llg/ml I0 llg/ml KB KB KB KB V20C KB7D KB V20C KB7D KB V20C KB7D 1 41.1 16.4 28.6 99.5 56.6 89.9 96.2 95.3 94.2 II 8.3 22.0 9.6 94.2 50.2 85.5 95.3 92 97.4 III 22.7 26.1 6.6 96.9 88.9 93.8 97.4 106.8 100.1 IV 42.5 27.3 6.1 98.5 105.6 95.1 97.9 100.3 98.5 l~Cell viability representsthe concentration IJ.Mof a compound required for 50% inhibition of cell growth. Login to comment 51 ABCC1 p.Val20Cys The MeOH extract of the roots of Aconitum pseudo-laeve var. erectum NAKAI (Ranunculaceae) showed significant modulating activity of Pgp-MDR against KB V20C but not MRP-MDR. Login to comment