ABCB3 p.Arg175His
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PMID: 10618714
[PubMed]
Zhu K et al: "p53 induces TAP1 and enhances the transport of MHC class I peptides."
No.
Sentence
Comment
89
In contrast, the luciferase activity for TAP1-Fluc was not increased by the mutants p53(V143A), p53(R175H), p53(R249S), or p53(R273H) (Figure 5c), consistent with the observation that mutant p53(R249S) was incapable of inducing TAP1 (Figure 1a).
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ABCB3 p.Arg175His 10618714:89:100
status: NEW95 5 mg of TAP1-Fluc was co-transfected into H1299 cells with 5 mg of pcDNA3 or a vector that expresses p53(V143A), p53(R175H), p53(R249S), or p53(R273H).
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ABCB3 p.Arg175His 10618714:95:117
status: NEW190 TAP1-Fluc or GADD45-Fluc was co-transfected into H1299 cells with control vector pcDNA3 or a vector that expresses wild-type p53, p53(V143A), p53(R175H), p53(R249S), p53(R273H), p73a or p73b.
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ABCB3 p.Arg175His 10618714:190:146
status: NEW
PMID: 12857899
[PubMed]
Herzer K et al: "Upregulation of major histocompatibility complex class I on liver cells by hepatitis C virus core protein via p53 and TAP1 impairs natural killer cell cytotoxicity."
No.
Sentence
Comment
177
Luciferase activity was not increased upon cotransfection of mutant p53(R175H), which contains a defect in the DNA-binding domain (Fig. 3C).
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ABCB3 p.Arg175His 12857899:177:72
status: NEW199 Hep3B and Huh7 cells (both p53afa; ) were cotransfected with TAP1-Luc, decreasing amounts of HCV core expression vector, the empty expression vector to adjust for equal amounts of DNA, WTp53, or p53(R175H), as indicated.
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ABCB3 p.Arg175His 12857899:199:202
status: NEW203 Hep3B and Huh7 cells were transfected with expression plasmids for WTp53, p53(R175H), or the HCV core protein alone or in combination as indicated.
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ABCB3 p.Arg175His 12857899:203:78
status: NEW208 served upregulation of TAP1, also MHC class I expression was increased in response to HCV core protein in the presence of WTp53 but not in the presence of p53(R175H) (Fig. 4).
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ABCB3 p.Arg175His 12857899:208:159
status: NEW333 Finally, we may be able to take advantage of this knowledge for vaccine development or specific immunotherapy. ACKNOWLEDGMENTS We are grateful to M. Oren for providing expression plasmids WTp53 and p53(R175H), to B. Vogelstein for pG13-Luc and tools for adenovirus constructs, to E. Klar and T. Lehnert for making human liver tissue available, to R. Bartenschlager and T. Pietschmann for providing antibodies, to D. Koppenho &#a8;fer, M. Pach, and B. Mosetter for excellent technical assistance, and to A. Krueger and M. Sprick for critical review of the manuscript.
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ABCB3 p.Arg175His 12857899:333:202
status: NEW