ABCMdb

PMID: 12857899

Herzer K, Falk CS, Encke J, Eichhorst ST, Ulsenheimer A, Seliger B, Krammer PH
Upregulation of major histocompatibility complex class I on liver cells by hepatitis C virus core protein via p53 and TAP1 impairs natural killer cell cytotoxicity.
J Virol. 2003 Aug;77(15):8299-309., [PubMed]

Sentences

No. Mutations Sentence Comment

177 ABCB3 p.Arg175His Luciferase activity was not increased upon cotransfection of mutant p53(R175H), which contains a defect in the DNA-binding domain (Fig. 3C). Login to comment 199 ABCB3 p.Arg175His Hep3B and Huh7 cells (both p53afa; ) were cotransfected with TAP1-Luc, decreasing amounts of HCV core expression vector, the empty expression vector to adjust for equal amounts of DNA, WTp53, or p53(R175H), as indicated. Login to comment 203 ABCB3 p.Arg175His Hep3B and Huh7 cells were transfected with expression plasmids for WTp53, p53(R175H), or the HCV core protein alone or in combination as indicated. Login to comment 208 ABCB3 p.Arg175His served upregulation of TAP1, also MHC class I expression was increased in response to HCV core protein in the presence of WTp53 but not in the presence of p53(R175H) (Fig. 4). Login to comment 333 ABCB3 p.Arg175His Finally, we may be able to take advantage of this knowledge for vaccine development or specific immunotherapy. ACKNOWLEDGMENTS We are grateful to M. Oren for providing expression plasmids WTp53 and p53(R175H), to B. Vogelstein for pG13-Luc and tools for adenovirus constructs, to E. Klar and T. Lehnert for making human liver tissue available, to R. Bartenschlager and T. Pietschmann for providing antibodies, to D. Koppenho &#a8;fer, M. Pach, and B. Mosetter for excellent technical assistance, and to A. Krueger and M. Sprick for critical review of the manuscript. Login to comment