ABCB3 p.Met285Cys
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PMID: 17164240
[PubMed]
Herget M et al: "Mechanism of substrate sensing and signal transmission within an ABC transporter: use of a Trojan horse strategy."
No.
Sentence
Comment
39
Expression of Human TAP Mutants-The single cysteine TAP1 mutants, Q277C, G282C, N283C, I284C, M285C, S286C, R287C, V288C, and R659C, were generated by ligase chain reaction with the following primers using cysteine-less human TAP1 with a C-terminal His10 tag as template (16): Q277C, CCGAATTCTTCCAGTGCAACCAGACCGC; G282C, GCA- GAACCAGACCTGCAACATCATGTCC; N283C, CAGAAC- CAGACCGGCTGCATCATGTCCAGAG; I284C, GACCGG- CAACTGCATGTCCAGAG; M285C, GACCGGCAACAT- CTGCTCCAGAGTCACCGAAG; S286C, GGCAACATCAT- GTGTAGAGTCACCGAAGA; R287C, GCAACATCATGTC- CTGCGTCACCGAAGATAC; V288C, CGGCAACATCAT- GTCCAGATGCACCGAAGATACG; and R659C, CAAGC- CTCTGCCTCAGTACG.
X
ABCB3 p.Met285Cys 17164240:39:94
status: NEWX
ABCB3 p.Met285Cys 17164240:39:429
status: NEW40 Expression of Human TAP Mutants-The single cysteine TAP1 mutants, Q277C, G282C, N283C, I284C, M285C, S286C, R287C, V288C, and R659C, were generated by ligase chain reaction with the following primers using cysteine-less human TAP1 with a C-terminal His10 tag as template (16): Q277C, CCGAATTCTTCCAGTGCAACCAGACCGC; G282C, GCA- GAACCAGACCTGCAACATCATGTCC; N283C, CAGAAC- CAGACCGGCTGCATCATGTCCAGAG; I284C, GACCGG- CAACTGCATGTCCAGAG; M285C, GACCGGCAACAT- CTGCTCCAGAGTCACCGAAG; S286C, GGCAACATCAT- GTGTAGAGTCACCGAAGA; R287C, GCAACATCATGTC- CTGCGTCACCGAAGATAC; V288C, CGGCAACATCAT- GTCCAGATGCACCGAAGATACG; and R659C, CAAGC- CTCTGCCTCAGTACG.
X
ABCB3 p.Met285Cys 17164240:40:94
status: NEWX
ABCB3 p.Met285Cys 17164240:40:429
status: NEW