ABCMdb

ABCB2 p.Thr98Gly

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Publications

PMID: 21520005 [PubMed] Zhang SY et al: "HMME-based PDT restores expression and function of transporter associated with antigen processing 1 (TAP1) and surface presentation of MHC class I antigen in human glioma."

No. Sentence Comment

48 Materials and methods Cells and reagents Three human glioma cell lines (U87, U251, and T98G) were obtained from the Animal House of Sun Yat-sen University (Guangzhou, China). Login to comment
86 In-vitro peptide transport assay Peptide transport in permeabilized U251, U87, and T98G cells was measured as described previously for EC and HeLa cells, with minor modifications [30]. Login to comment
109 Results Cytotoxic effects of PDT on human glioma cell lines To determine the cytotoxic effects of PDT on the growth of tumor cells, U251, U87, and T98G cells in the exponential growth phase were incubated for 24 h with 10 lg/mL HMME (the maximum non-toxic concentration). Login to comment
132 U251, U87, and T98G cells in exponential growth phase were incubated for 24 h with 10 lg/mL HMME (the maximum innocuous concentration). Login to comment
148 As shown in Fig. 4, untreated U251 cells (a), U87 cells (b) and T98G cells (c) all expressed barely detectable levels of TAP1. Login to comment
173 c TAP1 protein was detected in T98G cell lines. Login to comment
223 Three tumor cell lines (U251, U87, and T98G) were treated with HMME-PDT (2.4 J/cm2 ? Login to comment

PMID: 17541610 [PubMed] Mehling M et al: "WHO grade associated downregulation of MHC class I antigen-processing machinery components in human astrocytomas: does it reflect a potential immune escape mechanism?"

No. Sentence Comment

42 Cell lines The human glioma cell lines A172, D247MG, LN-18, LNT-229, LN-308, LN-319, LN-428, T98G, U87MG, U138MG, U251MG, and U373MG (kindly provided by Dr. N. de Tribolet, Neurosurgical Service, Centre Hospitalier Universitaire, Vaudois, Lausanne, Switzerland) were cultured in 75 cm3 Falcon plastic Xasks (BD Biosciences, Heidelberg, Germany) using DMEM supplemented with 1% glutamine (Life Technologies, Paisley, UK), 10% FCS (Biochrom, Berlin, Germany), and penicillin (100 IU/ml)/ streptomycin (100 g/ml) (Life Technologies) [49]. Login to comment

PMID: 16509500 [PubMed] Satoh E et al: "Reduced expression of the transporter associated with antigen processing 1 molecule in malignant glioma cells, and its restoration by interferon-gamma and -beta."

No. Sentence Comment

22 Materials and Methods Cell Culture, Tissue Samples, and Reagents Three human GBM cell lines (A172, U251, and T98G) and a mixed glioma cell line (KG1C) were obtained from the Japanese Cancer Resources Bank (Tokyo, Japan). Login to comment
77 Four glioma cell lines (A172, KG1C, YMG2, and YMG4) showed T and T in alleles, two cell lines (T98G and YMG5) G and T (Fig. 4), and two cell lines (U251 and YMG1) G and G. Login to comment
78 Four glioma cell lines (A172, KG1C, YMG2, and YMG4) showed T and T in alleles, two cell lines (T98G and YMG5) G and T (Fig. 4), and two cell lines (U251 and YMG1) G and G. Login to comment

PMID: 11807984 [PubMed] Morimoto I et al: "Identification of the osteopontin gene as a direct target of TP53."

No. Sentence Comment

28 Human tumor cell lines A172 (glioblastoma), T98G (glioblastoma), Saos2 (osteosarcoma), HCT116 (colorectal carcinoma), and H1299 (non-small-cell lung carcinoma) were purchased from the American Type Culture Collection (Rockville, MD). Login to comment
32 A172, T98G, and Saos2 cells were incubated continuously with adriamycin at 0.2 ␮g/mL. Login to comment

PMID: 23526983 [PubMed] Pan Y et al: "Rescue of MHC-1 antigen processing machinery by down-regulation in expression of IGF-1 in human glioblastoma cells."

No. Sentence Comment

218 Down-regulation in Expression of TAP-1 in pAnti IGF-1 Transfected HGB Cells by Exogenous Addition of IGF-1 Occurs with Inhibition of Phosphorylated STAT3(Tyr 705) and in Absence of Phosphorylated STAT1(Tyr 701) In order to determine whether Stat signaling is involved in the IGF-1 regulated expression of the TAP-1 gene, the pAnti IGF-1 transfected T98G and/or HG-2 cells were treated with addition of IGF-1 (100 ng/ml) in absence of serum and with ZnSO4 (50uM) added to culture medium. Login to comment
230 The expression of pStat1 in T98G NT (data not shown) and T98G TX cells was not detected; and it was also not detected after the exogenous addition of IGF-1 (Fig 7 B, C). Login to comment
231 In contrast, pStat3 was expressed in both T98G NT (data not shown) and T98G TX cells; and it was transiently decreased following addition of IGF-1 to T98G TX cells for a limited time interval (Fig 7 B, C). Login to comment
243 A, Regulation of TAP-1 peptide in T98G and HG-2 TX cells was determined by Western Blot. Login to comment
246 Lanes 1, 2 are NT cells from the T98G and HG-2 cell lines respectively. Lane 3, 4 are TX cells from clone b and c of the T98G cell line; Lane 5, 6 are TX cells from clone 5 and 11 of the HG-2 cell line. Login to comment
251 Lanes 1, 2 and 3 represent T98G, HG-2 and HG-9 cell lines cultured in medium with no IGF-1R mAb added, while lanes 4, 5 and 6 are the corresponding respective cell lines cultured in medium with addition of exogenous IGF-1R mAb (10ug/ml), respectively. Login to comment
253 Row 5 demonstrates the rescue in expression of the B-7.1 peptide in IGF-1R mAb treated T98G, HG-2 and HG-9 cell lines when compared to the wild types of the corresponding non-treated cells. Login to comment
263 In addition, and importantly, similar results were obtained using the ATCC T98G stably cloned cell line of Glioblastoma as a standard control. Login to comment
275 Different clones of transfected (TX) and corresponding non-transfected (NT) HGB cell lines, eight from T98G (circles), two from HG-2 (rectangles) and one from HG-3 (triangle), were examined by flow cytometry for the expression of TAP-1. Login to comment
279 T98G cells were transfected with the vector containing antisense IGF-1 cDNA (TX pAnti IGF-1), with vector containing antisense IGF-2 cDNA (TX pAnti IGF-2), or, with vector containing no antisense sequence (TX pEMT). Login to comment
280 Transfected cells were comparatively examined by flow cytometry relative to non-transfected T98G cells (NT) for content of TAP-1. Login to comment
290 Firstly, pSTAT 3 was detected in pAnti IGF-1 transfected, IGF-1 down-regulated T98G cells. Login to comment
292 These data show that STAT 3 may have a tumor-suppressive function in Glioblastoma cells, such as in the T98G cell line. Login to comment
298 PAkt was not detectable in pAnti IGF-1 transfected T98G cells cultured in serum-free medium plus 50 uM Zn2+. Login to comment
313 A, Expression of TAP-1 peptide in T98G cells was down-regulated by addition of IGF-1 to culture medium. Login to comment
315 Lanes 1 and 2 are NT cells from the T98G and HG-2 cell lines respectively. Lane 3 and lane 4 are T98G TX (clone b) and HG-2 TX (clone 5), respectively, into which IGF-1 was added overnight. Login to comment
318 The expression of TAP-1 was examined as a function of time following addition of IGF-1 (100 ng/ml) in TX T98G cells in absence of serum and in the presence of Zn2+. Login to comment