ABCMdb

PMID: 21520005

Zhang SY, Li JL, Xu XK, Zheng MG, Wen CC, Li FC
HMME-based PDT restores expression and function of transporter associated with antigen processing 1 (TAP1) and surface presentation of MHC class I antigen in human glioma.
J Neurooncol. 2011 Nov;105(2):199-210. Epub 2011 Apr 26., [PubMed]

Sentences

No. Mutations Sentence Comment

48 ABCB2 p.Thr98Gly Materials and methods Cells and reagents Three human glioma cell lines (U87, U251, and T98G) were obtained from the Animal House of Sun Yat-sen University (Guangzhou, China). Login to comment 86 ABCB2 p.Thr98Gly In-vitro peptide transport assay Peptide transport in permeabilized U251, U87, and T98G cells was measured as described previously for EC and HeLa cells, with minor modifications [30]. Login to comment 109 ABCB2 p.Thr98Gly Results Cytotoxic effects of PDT on human glioma cell lines To determine the cytotoxic effects of PDT on the growth of tumor cells, U251, U87, and T98G cells in the exponential growth phase were incubated for 24 h with 10 lg/mL HMME (the maximum non-toxic concentration). Login to comment 132 ABCB2 p.Thr98Gly U251, U87, and T98G cells in exponential growth phase were incubated for 24 h with 10 lg/mL HMME (the maximum innocuous concentration). Login to comment 148 ABCB2 p.Thr98Gly As shown in Fig. 4, untreated U251 cells (a), U87 cells (b) and T98G cells (c) all expressed barely detectable levels of TAP1. Login to comment 173 ABCB2 p.Thr98Gly c TAP1 protein was detected in T98G cell lines. Login to comment 223 ABCB2 p.Thr98Gly Three tumor cell lines (U251, U87, and T98G) were treated with HMME-PDT (2.4 J/cm2 ? Login to comment