ABCMdb

PMID: 23526983

Pan Y, Trojan J, Guo Y, Anthony DD
Rescue of MHC-1 antigen processing machinery by down-regulation in expression of IGF-1 in human glioblastoma cells.
PLoS One. 2013;8(3):e58428. doi: 10.1371/journal.pone.0058428. Epub 2013 Mar 20., [PubMed]

Sentences

No. Mutations Sentence Comment

218 ABCB2 p.Thr98Gly Down-regulation in Expression of TAP-1 in pAnti IGF-1 Transfected HGB Cells by Exogenous Addition of IGF-1 Occurs with Inhibition of Phosphorylated STAT3(Tyr 705) and in Absence of Phosphorylated STAT1(Tyr 701) In order to determine whether Stat signaling is involved in the IGF-1 regulated expression of the TAP-1 gene, the pAnti IGF-1 transfected T98G and/or HG-2 cells were treated with addition of IGF-1 (100 ng/ml) in absence of serum and with ZnSO4 (50uM) added to culture medium. Login to comment 230 ABCB2 p.Thr98Gly ABCB2 p.Thr98Gly The expression of pStat1 in T98G NT (data not shown) and T98G TX cells was not detected; and it was also not detected after the exogenous addition of IGF-1 (Fig 7 B, C). Login to comment 231 ABCB2 p.Thr98Gly ABCB2 p.Thr98Gly ABCB2 p.Thr98Gly In contrast, pStat3 was expressed in both T98G NT (data not shown) and T98G TX cells; and it was transiently decreased following addition of IGF-1 to T98G TX cells for a limited time interval (Fig 7 B, C). Login to comment 243 ABCB2 p.Thr98Gly A, Regulation of TAP-1 peptide in T98G and HG-2 TX cells was determined by Western Blot. Login to comment 246 ABCB2 p.Thr98Gly ABCB2 p.Thr98Gly Lanes 1, 2 are NT cells from the T98G and HG-2 cell lines respectively. Lane 3, 4 are TX cells from clone b and c of the T98G cell line; Lane 5, 6 are TX cells from clone 5 and 11 of the HG-2 cell line. Login to comment 251 ABCB2 p.Thr98Gly Lanes 1, 2 and 3 represent T98G, HG-2 and HG-9 cell lines cultured in medium with no IGF-1R mAb added, while lanes 4, 5 and 6 are the corresponding respective cell lines cultured in medium with addition of exogenous IGF-1R mAb (10ug/ml), respectively. Login to comment 253 ABCB2 p.Thr98Gly Row 5 demonstrates the rescue in expression of the B-7.1 peptide in IGF-1R mAb treated T98G, HG-2 and HG-9 cell lines when compared to the wild types of the corresponding non-treated cells. Login to comment 263 ABCB2 p.Thr98Gly In addition, and importantly, similar results were obtained using the ATCC T98G stably cloned cell line of Glioblastoma as a standard control. Login to comment 275 ABCB2 p.Thr98Gly Different clones of transfected (TX) and corresponding non-transfected (NT) HGB cell lines, eight from T98G (circles), two from HG-2 (rectangles) and one from HG-3 (triangle), were examined by flow cytometry for the expression of TAP-1. Login to comment 279 ABCB2 p.Thr98Gly T98G cells were transfected with the vector containing antisense IGF-1 cDNA (TX pAnti IGF-1), with vector containing antisense IGF-2 cDNA (TX pAnti IGF-2), or, with vector containing no antisense sequence (TX pEMT). Login to comment 280 ABCB2 p.Thr98Gly Transfected cells were comparatively examined by flow cytometry relative to non-transfected T98G cells (NT) for content of TAP-1. Login to comment 290 ABCB2 p.Thr98Gly Firstly, pSTAT 3 was detected in pAnti IGF-1 transfected, IGF-1 down-regulated T98G cells. Login to comment 292 ABCB2 p.Thr98Gly These data show that STAT 3 may have a tumor-suppressive function in Glioblastoma cells, such as in the T98G cell line. Login to comment 298 ABCB2 p.Thr98Gly PAkt was not detectable in pAnti IGF-1 transfected T98G cells cultured in serum-free medium plus 50 uM Zn2+. Login to comment 313 ABCB2 p.Thr98Gly A, Expression of TAP-1 peptide in T98G cells was down-regulated by addition of IGF-1 to culture medium. Login to comment 315 ABCB2 p.Thr98Gly ABCB2 p.Thr98Gly Lanes 1 and 2 are NT cells from the T98G and HG-2 cell lines respectively. Lane 3 and lane 4 are T98G TX (clone b) and HG-2 TX (clone 5), respectively, into which IGF-1 was added overnight. Login to comment 318 ABCB2 p.Thr98Gly The expression of TAP-1 was examined as a function of time following addition of IGF-1 (100 ng/ml) in TX T98G cells in absence of serum and in the presence of Zn2+. Login to comment