ABCMdb

ABCC1 p.Ser288Cys

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Publications

PMID: 21078121 [PubMed] Delalande O et al: "Cadmium-glutathione solution structures provide new insights into heavy metal detoxification."

No. Sentence Comment

176 Experimental procedures Strains and culture conditions The Saccharomyces cerevisiae strain used for the production of 15 N-GSH was S288C (Mata SUC2 mal mel gal2 CUP1). Login to comment
185 This extract contained soluble yeast metabolites, including 15 N-GSH (S288C strain) and 15 Nc-Glu-Cys (Dgsh2 strain). Login to comment
206 Strain No treatment 100 lM cadmium S288C 4.4 ± 0.3% 5.9 ± 0.8% BY4742 4.4 ± 0.8% 8.7 ± 0.3% resonances through TOCSY and off-resonance ROESY experiments. Login to comment

PMID: 1588958 [PubMed] Stohl LL et al: "Saccharomyces cerevisiae contains an RNase MRP that cleaves at a conserved mitochondrial RNA sequence implicated in replication priming."

No. Sentence Comment

65 S. cerevisiae strains used in this study were wild-type strains MH41-7B (a ade his [rho+]), S288C (obtained from the American Type Culture Collection stock center), and BJ2168 (a pep4-3 trpl) (obtained from T. Lisowsky); [rhoo] tester strainD243-4A 18 (a adel lys2, [rho°]) (obtained from T. Lisowsky); and a strain containing a disrupted mtRNA polymerase gene (rpo4l:: Tn:URA3), whose construction has been described in detail elsewhere (15). Login to comment
241 Lanes: M, HpaII-digested pBR322 DNA; 1, minus-enzyme control; 2 and 3, 1 and 5 p.l of extract from strain MH41-7B; 4 and 5, 1 and 5 pil of extract from strain S288C; 6 and 7, 1 and 5 pul of extract from the strain containing a disrupted mtRNA polymerase gene (15); 8 and 9, 1 and 5 pll of extract from [rhoo] tester strain D243-4A 18. indicating that the observed inactivation is not due to the presence oT an inhibitor generated during micrococcal nuclease digestion. Login to comment
62 S. cerevisiae strains used in this study were wild-type strains MH41-7B (a ade his [rho+]), S288C (obtained from the American Type Culture Collection stock center), and BJ2168 (a pep4-3 trpl) (obtained from T. Lisowsky); [rhoo] tester strain D243-4A 18 (a adel lys2, [rho&#b0;]) (obtained from T. Lisowsky); and a strain containing a disrupted mtRNA polymerase gene (rpo4l:: Tn:URA3), whose construction has been described in detail elsewhere (15). Login to comment
240 Lanes: M, HpaII-digested pBR322 DNA; 1, minus-enzyme control; 2 and 3, 1 and 5 p.l of extract from strain MH41-7B; 4 and 5, 1 and 5 pil of extract from strain S288C; 6 and 7, 1 and 5 pul of extract from the strain containing a disrupted mtRNA polymerase gene (15); 8 and 9, 1 and 5 pll of extract from [rhoo] tester strain D243-4A 18. indicating that the observed inactivation is not due to the presence oT an inhibitor generated during micrococcal nuclease digestion. Login to comment

PMID: 12796304 [PubMed] Mason DL et al: "A region within a lumenal loop of Saccharomyces cerevisiae Ycf1p directs proteolytic processing and substrate specificity."

No. Sentence Comment

203 We also examined the cadmium and arsenite resistance of the Ycf1p mutant proteins expressed from the chromosome in a different strain background, BY4741, which is the parental strain for the yeast knockout collection and a derivative of the strain S288C (17) (Fig. 6). Login to comment
214 We hypothesized that the nonsense codon might represent a mutation in S288C (the strain used to sequence the genome and the strain from which BY4741 is derived), whereas in SM1058 (also known as EG123 and whose lineage is distinct from S288C [40]), YKR103/104w may actually code a single "full-length" MRP-type transporter homologous throughout its length to Ycf1p and Bpt1p (6). Login to comment
216 To examine the possibility that YKR103/104w varies between strains, we used PCR amplification of genomic DNA, followed by DNA sequence analysis to confirm that, in the S288C- FIG. 5. Login to comment
224 Interestingly, other S. cerevisiae strains, whose lineages derive independently of S288C (SK1 and éa;1278b), and other species of yeast from the wild (S. paradoxus and S. mikatae), also encode the full-length NFT1 gene (Fig. 7B). Login to comment
268 In the course of our Ycf1p studies, we used two differing strain backgrounds: (i) SM1058, also designated EG123, which is our standard laboratory strain and has a poorly defined lineage (40), and (ii) BY4741, an S288C derivative that is the parental strain for the yeast deletion collection (17). Login to comment
277 The sequence deposited in SGD for S288C and our sequence results for BY4741 both contain a stop codon (*) at amino acid 1219, whereas other S. cerevisiae strains, as well as additional "wild" yeast strains, contain a tyrosine (Y) and thus code for a full-length ABC transporter. Login to comment
289 We discovered that in the strain SM1058, YKR103/104w is one continuous ORF rather than two ORFs separated by a nonsense codon, as in the strains BY4741 and S288C (Fig. 7). Login to comment
291 Interestingly, possession of a full-length NFT1 gene appears to be the "wild-type" situation for yeast, since other S. cerevisiae laboratory strains (éa;1278b and SK1) that differ in lineage from S288C also possess the full-length NFT1 gene, as do other Saccharomyces species (S. paradoxus and S. mikitae). Login to comment
292 Differences between S288C and other S. cerevisiae strains, as well as other Saccharomyces species, have been reported for a variety of genes, such as the aquaporin genes (9). Login to comment
293 It is possible that the manner in which S288C was cultivated in the laboratory led to an unintentional selection against the presence of full-length NFT1. Login to comment