ABCMdb

PMID: 1588958

Stohl LL, Clayton DA
Saccharomyces cerevisiae contains an RNase MRP that cleaves at a conserved mitochondrial RNA sequence implicated in replication priming.
Mol Cell Biol. 1992 Jun;12(6):2561-9., [PubMed]

Sentences

No. Mutations Sentence Comment

62 ABCC1 p.Ser288Cys S. cerevisiae strains used in this study were wild-type strains MH41-7B (a ade his [rho+]), S288C (obtained from the American Type Culture Collection stock center), and BJ2168 (a pep4-3 trpl) (obtained from T. Lisowsky); [rhoo] tester strain D243-4A 18 (a adel lys2, [rho&#b0;]) (obtained from T. Lisowsky); and a strain containing a disrupted mtRNA polymerase gene (rpo4l:: Tn:URA3), whose construction has been described in detail elsewhere (15). Login to comment 65 ABCC1 p.Ser288Cys S. cerevisiae strains used in this study were wild-type strains MH41-7B (a ade his [rho+]), S288C (obtained from the American Type Culture Collection stock center), and BJ2168 (a pep4-3 trpl) (obtained from T. Lisowsky); [rhoo] tester strainD243-4A 18 (a adel lys2, [rho°]) (obtained from T. Lisowsky); and a strain containing a disrupted mtRNA polymerase gene (rpo4l:: Tn:URA3), whose construction has been described in detail elsewhere (15). Login to comment 240 ABCC1 p.Ser288Cys Lanes: M, HpaII-digested pBR322 DNA; 1, minus-enzyme control; 2 and 3, 1 and 5 p.l of extract from strain MH41-7B; 4 and 5, 1 and 5 pil of extract from strain S288C; 6 and 7, 1 and 5 pul of extract from the strain containing a disrupted mtRNA polymerase gene (15); 8 and 9, 1 and 5 pll of extract from [rhoo] tester strain D243-4A 18. indicating that the observed inactivation is not due to the presence oT an inhibitor generated during micrococcal nuclease digestion. Login to comment 241 ABCC1 p.Ser288Cys Lanes: M, HpaII-digested pBR322 DNA; 1, minus-enzyme control; 2 and 3, 1 and 5 p.l of extract from strain MH41-7B; 4 and 5, 1 and 5 pil of extract from strain S288C; 6 and 7, 1 and 5 pul of extract from the strain containing a disrupted mtRNA polymerase gene (15); 8 and 9, 1 and 5 pll of extract from [rhoo] tester strain D243-4A 18. indicating that the observed inactivation is not due to the presence oT an inhibitor generated during micrococcal nuclease digestion. Login to comment