ABCMdb

PMID: 12796304

Mason DL, Mallampalli MP, Huyer G, Michaelis S
A region within a lumenal loop of Saccharomyces cerevisiae Ycf1p directs proteolytic processing and substrate specificity.
Eukaryot Cell. 2003 Jun;2(3):588-98., [PubMed]

Sentences

No. Mutations Sentence Comment

203 ABCC1 p.Ser288Cys We also examined the cadmium and arsenite resistance of the Ycf1p mutant proteins expressed from the chromosome in a different strain background, BY4741, which is the parental strain for the yeast knockout collection and a derivative of the strain S288C (17) (Fig. 6). Login to comment 214 ABCC1 p.Ser288Cys ABCC1 p.Ser288Cys We hypothesized that the nonsense codon might represent a mutation in S288C (the strain used to sequence the genome and the strain from which BY4741 is derived), whereas in SM1058 (also known as EG123 and whose lineage is distinct from S288C [40]), YKR103/104w may actually code a single "full-length" MRP-type transporter homologous throughout its length to Ycf1p and Bpt1p (6). Login to comment 216 ABCC1 p.Ser288Cys To examine the possibility that YKR103/104w varies between strains, we used PCR amplification of genomic DNA, followed by DNA sequence analysis to confirm that, in the S288C- FIG. 5. Login to comment 224 ABCC1 p.Ser288Cys Interestingly, other S. cerevisiae strains, whose lineages derive independently of S288C (SK1 and éa;1278b), and other species of yeast from the wild (S. paradoxus and S. mikatae), also encode the full-length NFT1 gene (Fig. 7B). Login to comment 268 ABCC1 p.Ser288Cys In the course of our Ycf1p studies, we used two differing strain backgrounds: (i) SM1058, also designated EG123, which is our standard laboratory strain and has a poorly defined lineage (40), and (ii) BY4741, an S288C derivative that is the parental strain for the yeast deletion collection (17). Login to comment 277 ABCC1 p.Ser288Cys The sequence deposited in SGD for S288C and our sequence results for BY4741 both contain a stop codon (*) at amino acid 1219, whereas other S. cerevisiae strains, as well as additional "wild" yeast strains, contain a tyrosine (Y) and thus code for a full-length ABC transporter. Login to comment 289 ABCC1 p.Ser288Cys We discovered that in the strain SM1058, YKR103/104w is one continuous ORF rather than two ORFs separated by a nonsense codon, as in the strains BY4741 and S288C (Fig. 7). Login to comment 291 ABCC1 p.Ser288Cys Interestingly, possession of a full-length NFT1 gene appears to be the "wild-type" situation for yeast, since other S. cerevisiae laboratory strains (éa;1278b and SK1) that differ in lineage from S288C also possess the full-length NFT1 gene, as do other Saccharomyces species (S. paradoxus and S. mikitae). Login to comment 292 ABCC1 p.Ser288Cys Differences between S288C and other S. cerevisiae strains, as well as other Saccharomyces species, have been reported for a variety of genes, such as the aquaporin genes (9). Login to comment 293 ABCC1 p.Ser288Cys It is possible that the manner in which S288C was cultivated in the laboratory led to an unintentional selection against the presence of full-length NFT1. Login to comment