ABCC2 p.Gly418Arg
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PMID: 9334225
[PubMed]
Kast C et al: "Topology mapping of the amino-terminal half of multidrug resistance-associated protein by epitope insertion and immunofluorescence."
No.
Sentence
Comment
69
Mass populations of G418R transfectants were harvested after 9 days and subcultured in medium containing the VP-16 (final concentration 250 ng/ml) to select mass populations of drug-resistant cell clones overexpressing the tagged MRP proteins.
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ABCC2 p.Gly418Arg 9334225:69:20
status: ERRONEOUS109 Stable transfectants were selected in G418 and mass populations of G418R colonies were further selected in VP-16 (250 ng/ml).
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ABCC2 p.Gly418Arg 9334225:109:67
status: ERRONEOUS
PMID: 9485377
[PubMed]
Kast C et al: "Epitope insertion favors a six transmembrane domain model for the carboxy-terminal portion of the multidrug resistance-associated protein."
No.
Sentence
Comment
65
Mass populations of G418R transfectants were harvested after 9 days and subcultured in medium containing the etoposide VP-16 (final concentration 250 ng/mL) to select mass populations of drug-resistant cell clones overexpressing the tagged MRP proteins.
X
ABCC2 p.Gly418Arg 9485377:65:20
status: NEW105 Stable transfectants were selected in G418, and mass populations of G418R colonies were further selected in VP-16 (250 ng/ mL).
X
ABCC2 p.Gly418Arg 9485377:105:68
status: NEW106 Drug-resistant colonies emerged within 1-2 weeks of selection in populations of G418R cells transfected with wild-type MRP or with MRP constructs 1, 2a, 2b, 5a, and Table 1: Oligonucleotides Used for Epitope Insertion by Site-Directed Mutagenesis 5b.
X
ABCC2 p.Gly418Arg 9485377:106:80
status: NEW