PMID: 9485377

Kast C, Gros P
Epitope insertion favors a six transmembrane domain model for the carboxy-terminal portion of the multidrug resistance-associated protein.
Biochemistry. 1998 Feb 24;37(8):2305-13., [PubMed]
Sentences
No. Mutations Sentence Comment
65 ABCC2 p.Gly418Arg
X
ABCC2 p.Gly418Arg 9485377:65:20
status: NEW
view ABCC2 p.Gly418Arg details
 Mass populations of G418R transfectants were harvested after 9 days and subcultured in medium containing the etoposide VP-16 (final concentration 250 ng/mL) to select mass populations of drug-resistant cell clones overexpressing the tagged MRP proteins. Login to comment 105 ABCC2 p.Gly418Arg
X
ABCC2 p.Gly418Arg 9485377:105:68
status: NEW
view ABCC2 p.Gly418Arg details
 Stable transfectants were selected in G418, and mass populations of G418R colonies were further selected in VP-16 (250 ng/ mL). Login to comment 106 ABCC2 p.Gly418Arg
X
ABCC2 p.Gly418Arg 9485377:106:80
status: NEW
view ABCC2 p.Gly418Arg details
 Drug-resistant colonies emerged within 1-2 weeks of selection in populations of G418R cells transfected with wild-type MRP or with MRP constructs 1, 2a, 2b, 5a, and Table 1: Oligonucleotides Used for Epitope Insertion by Site-Directed Mutagenesis 5b. Login to comment