ABCMdb

PMID: 9334225

Kast C, Gros P
Topology mapping of the amino-terminal half of multidrug resistance-associated protein by epitope insertion and immunofluorescence.
J Biol Chem. 1997 Oct 17;272(42):26479-87., [PubMed]

Sentences

No. Mutations Sentence Comment

63 ABCC1 p.Gly5Glu Cell Culture-Drug-sensitive human HeLa cells (ATCC) were grown in Dulbecco`s modified Eagle`s medium (high glucose) supplemented TABLE I Oligonucleotides used for epitope insertion by site-directed mutagenesis 1 Arg-4b (HA epitope)c Gly-5 Glu-127 (HA epitope) Arg-128 2 (363)a C TTT TTA ATT CAG CTG GAG AGG AGG AAG GGA GTT CAG (399) Glu-163 (HA epitope) Asp-164 3 (474) G ACA GCC TTA AAA GAG GAT GCC CAG GTG GAC (504) Gln-271 (HA epitope) Pro-272 4 (799) AAG ACTD AGG AAG CAG CCG GTG AAG GTT GTG TA (830) Ser-417 (HA epitope) Thr-419 5 (1233) C AAT TCA GCC AGA AAA TCC ACG GTC GGG GAG ATT G (1270) Asn-461 (HA epitope) Leu-462 6a (1369) CTC CTG TGG CTG AAT CTG GGC CCT TCC GTC (1398) 6b Asn-461 (HA epitope) ϫ 2 Leu-462 Leu-529 (HA epitope) Ala-530 7 (1572) C AAG GAC AAG GTG CTG GCT ATC AGG CAG GAG GA (1604) Asn-574 (HA epitope) Asn-575 8a (1707) G ACC ATT GAC GAG AAC AAT ATC CTG GAT GCC CA (1739) 8b Asn-574 (HA epitope) ϫ 2 Asn-575 9 Trp-653 (HA epitope) Ala-654 (HA epitope)c :Y P Y D V P D Y A S a Nucleotide position in sequence of MRP published by Cole et al. (10; 55). Login to comment 69 ABCC2 p.Gly418Arg Mass populations of G418R transfectants were harvested after 9 days and subcultured in medium containing the VP-16 (final concentration 250 ng/ml) to select mass populations of drug-resistant cell clones overexpressing the tagged MRP proteins. Login to comment 109 ABCC2 p.Gly418Arg Stable transfectants were selected in G418 and mass populations of G418R colonies were further selected in VP-16 (250 ng/ml). Login to comment