ABCB1 p.Ser661Ala

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PMID: 9287320 [PubMed] Szabo K et al: "Phosphorylation site mutations in the human multidrug transporter modulate its drug-stimulated ATPase activity."
No. Sentence Comment
30 1 The abbreviations used are: MDR1, human multidrug resistance protein; 3A, mutant MDR1 with mutations S661A, S667A, and S671A; 3E, mutant MDR1 with mutations S661E, S667E, and S671E; 8A, mutant MDR1 with mutations S660A, S661A, S667A, T668A, S671A, S675A, S683A, and T684A; 8E, mutant MDR1 with mutations S660E, S661E, S667E, T668E, S671E, S675E, S683E, and T684E; AM, acetoxymethylester; Sf9, Spodoptera frugiperda ovarian cells.
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ABCB1 p.Ser661Ala 9287320:30:103
status: NEW
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ABCB1 p.Ser661Ala 9287320:30:222
status: NEW
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40 Baculovirus transfer vectors were constructed by using the human MDR1 cDNA encoding a protein with the following mutants: S661A, S667A, and S671A (3A); S661E, S667E, and S671E (3E); S660A, S661A, S667A, T668A, S671A, S675A, S683A, and T684A (8A); and S660E, S661E, S667E, T668E, S671E, S675E, S683E, and T684E (8E), as described earlier (22, 32).
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ABCB1 p.Ser661Ala 9287320:40:122
status: NEW
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ABCB1 p.Ser661Ala 9287320:40:189
status: NEW
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PMID: 10571246 [PubMed] Castro AF et al: "Mechanism of inhibition of P-glycoprotein-mediated drug transport by protein kinase C blockers."
No. Sentence Comment
50 The primary screening of the Ser661 to Ala and Ser667 to Ala mutants was done with HinP1 I (site added) and DpnI (site removed), respectively.
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ABCB1 p.Ser661Ala 10571246:50:29
status: NEW
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46 The primary screening of the Ser661 to Ala and Ser667 to Ala mutants was done with HinP1 I (site added) and DpnI (site removed), respectively.
X
ABCB1 p.Ser661Ala 10571246:46:29
status: NEW
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