ABCMdb

PMID: 10571246

Castro AF, Horton JK, Vanoye CG, Altenberg GA
Mechanism of inhibition of P-glycoprotein-mediated drug transport by protein kinase C blockers.
Biochem Pharmacol. 1999 Dec 1;58(11):1723-33., [PubMed]

Sentences

No. Mutations Sentence Comment

43 ABCB1 p.Ser667Ala ABCB1 p.Ser671Ala The mutagenic oligonucleotides were: 5b18;-TTCAAGATCCGCGCTAATAAGAA-3b18; (Ser [661] to Ala), 5b18;-AAGAAAAAGAGCCACTCGT- AGG-3b18; (Ser667 to Ala), and 5b18;-ACTCGTAGAGCGG- TCCGTGGATC-3b18; (Ser671 to Ala). Login to comment 45 ABCB1 p.Ser671Ala The Ser671 to Ala mutagenic oligonucleotide includes one additional base mutation (silent) that adds a BsrBI site for easy screening. Login to comment 46 ABCB1 p.Ser667Ala ABCB1 p.Ser661Ala The primary screening of the Ser661 to Ala and Ser667 to Ala mutants was done with HinP1 I (site added) and DpnI (site removed), respectively. Login to comment 47 ABCB1 p.Ser667Ala ABCB1 p.Ser671Ala The mutagenic oligonucleotides were: 5Ј-TTCAAGATCCGCGCTAATAAGAA-3Ј (Ser [661] to Ala), 5Ј-AAGAAAAAGAGCCACTCGT- AGG-3Ј (Ser667 to Ala), and 5Ј-ACTCGTAGAGCGG- TCCGTGGATC-3Ј (Ser671 to Ala). Login to comment 49 ABCB1 p.Ser671Ala The Ser671 to Ala mutagenic oligonucleotide includes one additional base mutation (silent) that adds a BsrBI site for easy screening. Login to comment 50 ABCB1 p.Ser667Ala ABCB1 p.Ser661Ala The primary screening of the Ser661 to Ala and Ser667 to Ala mutants was done with HinP1 I (site added) and DpnI (site removed), respectively. Login to comment