ABCMdb

PMID: 9287320

Szabo K, Bakos E, Welker E, Muller M, Goodfellow HR, Higgins CF, Varadi A, Sarkadi B
Phosphorylation site mutations in the human multidrug transporter modulate its drug-stimulated ATPase activity.
J Biol Chem. 1997 Sep 12;272(37):23165-71., 1997-09-12 [PubMed]

Sentences

No. Mutations Sentence Comment

30 ABCB1 p.Thr668Ala ABCB1 p.Ser675Glu ABCB1 p.Ser667Glu ABCB1 p.Ser667Glu ABCB1 p.Ser660Ala ABCB1 p.Thr668Glu ABCB1 p.Ser675Ala ABCB1 p.Ser667Ala ABCB1 p.Ser667Ala ABCB1 p.Ser660Glu ABCB1 p.Ser661Glu ABCB1 p.Ser661Glu ABCB1 p.Ser671Ala ABCB1 p.Ser671Ala ABCB1 p.Thr684Ala ABCB1 p.Ser661Ala ABCB1 p.Ser661Ala ABCB1 p.Ser671Glu ABCB1 p.Ser671Glu ABCB1 p.Thr684Glu ABCB1 p.Ser683Ala ABCB1 p.Ser683Glu 1 The abbreviations used are: MDR1, human multidrug resistance protein; 3A, mutant MDR1 with mutations S661A, S667A, and S671A; 3E, mutant MDR1 with mutations S661E, S667E, and S671E; 8A, mutant MDR1 with mutations S660A, S661A, S667A, T668A, S671A, S675A, S683A, and T684A; 8E, mutant MDR1 with mutations S660E, S661E, S667E, T668E, S671E, S675E, S683E, and T684E; AM, acetoxymethylester; Sf9, Spodoptera frugiperda ovarian cells. Login to comment 40 ABCB1 p.Thr668Ala ABCB1 p.Ser675Glu ABCB1 p.Ser667Glu ABCB1 p.Ser667Glu ABCB1 p.Ser660Ala ABCB1 p.Thr668Glu ABCB1 p.Ser675Ala ABCB1 p.Ser667Ala ABCB1 p.Ser667Ala ABCB1 p.Ser660Glu ABCB1 p.Ser661Glu ABCB1 p.Ser661Glu ABCB1 p.Ser671Ala ABCB1 p.Ser671Ala ABCB1 p.Thr684Ala ABCB1 p.Ser661Ala ABCB1 p.Ser661Ala ABCB1 p.Ser671Glu ABCB1 p.Ser671Glu ABCB1 p.Thr684Glu ABCB1 p.Ser683Ala ABCB1 p.Ser683Glu Baculovirus transfer vectors were constructed by using the human MDR1 cDNA encoding a protein with the following mutants: S661A, S667A, and S671A (3A); S661E, S667E, and S671E (3E); S660A, S661A, S667A, T668A, S671A, S675A, S683A, and T684A (8A); and S660E, S661E, S667E, T668E, S671E, S675E, S683E, and T684E (8E), as described earlier (22, 32). Login to comment 158 ABCB1 p.Ser671Asn Ahmad et al. (40) have also presented evidence that exchanging Ser671 to asparagine reduced ATPase activity by about 50% at 10 ␮M verapamil when expressed in Sf9 cells. Login to comment