ABCB1 p.Ser667Ala
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PMID: 9287320
[PubMed]
Szabo K et al: "Phosphorylation site mutations in the human multidrug transporter modulate its drug-stimulated ATPase activity."
No.
Sentence
Comment
30
1 The abbreviations used are: MDR1, human multidrug resistance protein; 3A, mutant MDR1 with mutations S661A, S667A, and S671A; 3E, mutant MDR1 with mutations S661E, S667E, and S671E; 8A, mutant MDR1 with mutations S660A, S661A, S667A, T668A, S671A, S675A, S683A, and T684A; 8E, mutant MDR1 with mutations S660E, S661E, S667E, T668E, S671E, S675E, S683E, and T684E; AM, acetoxymethylester; Sf9, Spodoptera frugiperda ovarian cells.
X
ABCB1 p.Ser667Ala 9287320:30:110
status: NEWX
ABCB1 p.Ser667Ala 9287320:30:229
status: NEW40 Baculovirus transfer vectors were constructed by using the human MDR1 cDNA encoding a protein with the following mutants: S661A, S667A, and S671A (3A); S661E, S667E, and S671E (3E); S660A, S661A, S667A, T668A, S671A, S675A, S683A, and T684A (8A); and S660E, S661E, S667E, T668E, S671E, S675E, S683E, and T684E (8E), as described earlier (22, 32).
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ABCB1 p.Ser667Ala 9287320:40:129
status: NEWX
ABCB1 p.Ser667Ala 9287320:40:196
status: NEW
PMID: 10571246
[PubMed]
Castro AF et al: "Mechanism of inhibition of P-glycoprotein-mediated drug transport by protein kinase C blockers."
No.
Sentence
Comment
47
The mutagenic oligonucleotides were: 5Ј-TTCAAGATCCGCGCTAATAAGAA-3Ј (Ser [661] to Ala), 5Ј-AAGAAAAAGAGCCACTCGT- AGG-3Ј (Ser667 to Ala), and 5Ј-ACTCGTAGAGCGG- TCCGTGGATC-3Ј (Ser671 to Ala).
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ABCB1 p.Ser667Ala 10571246:47:143
status: NEW50 The primary screening of the Ser661 to Ala and Ser667 to Ala mutants was done with HinP1 I (site added) and DpnI (site removed), respectively.
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ABCB1 p.Ser667Ala 10571246:50:47
status: NEW43 The mutagenic oligonucleotides were: 5b18;-TTCAAGATCCGCGCTAATAAGAA-3b18; (Ser [661] to Ala), 5b18;-AAGAAAAAGAGCCACTCGT- AGG-3b18; (Ser667 to Ala), and 5b18;-ACTCGTAGAGCGG- TCCGTGGATC-3b18; (Ser671 to Ala).
X
ABCB1 p.Ser667Ala 10571246:43:143
status: NEW46 The primary screening of the Ser661 to Ala and Ser667 to Ala mutants was done with HinP1 I (site added) and DpnI (site removed), respectively.
X
ABCB1 p.Ser667Ala 10571246:46:47
status: NEW