ABCB1 p.Lys48Arg
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PMID: 15322230
[PubMed]
Zhang Z et al: "Regulation of the stability of P-glycoprotein by ubiquitination."
No.
Sentence
Comment
66
Plasmids PCW7 (wild-type ubiquitin) and PCW8 (ubiquitin K48R mutant) were kindly provided by Dr. Ron Kopito (Stanford University, Stanford, CA).
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ABCB1 p.Lys48Arg 15322230:66:56
status: NEW85 To determine the effect of ubiquitination on P-gp stability, we transiently transfected the MDR MCF-7 cells with a wild-type ubiquitin plasmid, PCW7, or a dominant-negative ubiquitin, PCW8 (K48R mutant), in which the invariant lysine at position 48 was replaced by arginine (Finley et al., 1994).
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ABCB1 p.Lys48Arg 15322230:85:190
status: NEWX
ABCB1 p.Lys48Arg 15322230:85:227
status: NEW87 The K48R ubiquitin mutant (PCW8) produces ubiquitin chain termination and accumulation of incompletely ubiquitinated proteins that are not targeted for proteasomal degradation (Ward et al., 1995; Yu and Kopito, 1999).
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ABCB1 p.Lys48Arg 15322230:87:4
status: NEW128 MDR MCF-7 cells were transfected with wild-type ubiquitin (A and B) or mutant ubiquitin K48R (C).
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ABCB1 p.Lys48Arg 15322230:128:88
status: NEW198 Acknowledgments We are grateful to Dr. Ron Kopito (Stanford University) for donating the plasmids PCW7 (wild-type ubiquitin) and PCW8 (ubiquitin K48R mutant), and to Dr. Michael Gottesman (National Cancer Institute) for providing the NIH3T3, N3V2400, N4V600, and N5V2400 cell lines.
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ABCB1 p.Lys48Arg 15322230:198:145
status: NEW
PMID: 2573836
[PubMed]
Azzaria M et al: "Discrete mutations introduced in the predicted nucleotide-binding sites of the mdr1 gene abolish its ability to confer multidrug resistance."
No.
Sentence
Comment
410
Mutation of lysine48 to arginine in the yeast RAD3 protein abolishes its ATPase and DNA helicase activities but not the ability to bind ATP.
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ABCB1 p.Lys48Arg 2573836:410:12
status: NEW