ABCMdb

PMID: 15322230

Zhang Z, Wu JY, Hait WN, Yang JM
Regulation of the stability of P-glycoprotein by ubiquitination.
Mol Pharmacol. 2004 Sep;66(3):395-403., [PubMed]

Sentences

No. Mutations Sentence Comment

66 ABCB1 p.Lys48Arg Plasmids PCW7 (wild-type ubiquitin) and PCW8 (ubiquitin K48R mutant) were kindly provided by Dr. Ron Kopito (Stanford University, Stanford, CA). Login to comment 85 ABCB1 p.Lys48Arg ABCB1 p.Lys48Arg To determine the effect of ubiquitination on P-gp stability, we transiently transfected the MDR MCF-7 cells with a wild-type ubiquitin plasmid, PCW7, or a dominant-negative ubiquitin, PCW8 (K48R mutant), in which the invariant lysine at position 48 was replaced by arginine (Finley et al., 1994). Login to comment 87 ABCB1 p.Lys48Arg The K48R ubiquitin mutant (PCW8) produces ubiquitin chain termination and accumulation of incompletely ubiquitinated proteins that are not targeted for proteasomal degradation (Ward et al., 1995; Yu and Kopito, 1999). Login to comment 128 ABCB1 p.Lys48Arg MDR MCF-7 cells were transfected with wild-type ubiquitin (A and B) or mutant ubiquitin K48R (C). Login to comment 198 ABCB1 p.Lys48Arg Acknowledgments We are grateful to Dr. Ron Kopito (Stanford University) for donating the plasmids PCW7 (wild-type ubiquitin) and PCW8 (ubiquitin K48R mutant), and to Dr. Michael Gottesman (National Cancer Institute) for providing the NIH3T3, N3V2400, N4V600, and N5V2400 cell lines. Login to comment