ABCC7 p.Leu1480Ala
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PMID: 11158634
[PubMed]
Raghuram V et al: "Regulation of cystic fibrosis transmembrane conductance regulator single-channel gating by bivalent PDZ-domain-mediated interaction."
No.
Sentence
Comment
61
[35 S]methionine-labeled full-length CFTR and CFTR-L1480A (Leu-1480 was replaced by Ala) was prepared by using an SP6 TnT (Promega) kit in the presence of canine pancreatic microsomes (Promega).
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ABCC7 p.Leu1480Ala 11158634:61:51
status: NEWX
ABCC7 p.Leu1480Ala 11158634:61:59
status: NEW110 The mutation L1480A resulted in a complete loss of interaction, and mutations T1378A, L1480V, and L1480M resulted in markedly reduced interaction (Fig. 1D), with both PDZ1 and PDZ2 having similar specificities for their target ligands.
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ABCC7 p.Leu1480Ala 11158634:110:13
status: NEW112 However, there are no additional PDZ binding sites in CFTR, because the PDZ1-2 domains bound to full-length in vitro-translated CFTR but not to mutant CFTR (L1480A) (Fig. 1C).
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ABCC7 p.Leu1480Ala 11158634:112:157
status: NEW142 [35S]methionine-labeled in vitro-translated products (I) and the GST-PDZ1-2 (1-299) ''pull-down`` products (P) of wild-type CFTR (WT) and mutant CFTR (L1480A) are indicated.
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ABCC7 p.Leu1480Ala 11158634:142:151
status: NEW
PMID: 11051556
[PubMed]
Wang S et al: "Accessory protein facilitated CFTR-CFTR interaction, a molecular mechanism to potentiate the chloride channel activity."
No.
Sentence
Comment
113
The amino acid substitution of leucine with alanine at the C-terminal end (L1480A) was designated as HA-CFTR-Ct*.
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ABCC7 p.Leu1480Ala 11051556:113:75
status: NEW157 (f and g) Open probability (Po) and mean opening time (MOT) of the wild-type CFTR and mutated CFTR-L1480A are plotted against different concentrations of purified recombinant CAP70 protein: filled squares, wild-type CFTR plus CAP70; filled circles, CFTR (L1480A) plus CAP70; open circles, wild-type CFTR plus PDZ3-3.
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ABCC7 p.Leu1480Ala 11051556:157:99
status: NEWX
ABCC7 p.Leu1480Ala 11051556:157:255
status: NEW159 n for wild-type CFTR is 5d51;9, for CFTR/L1480A is 4 and for PDZ3-3 is 5.
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ABCC7 p.Leu1480Ala 11051556:159:44
status: NEW212 substitution of the last residue leucine with alanine these possibilities, we tested the effect of a tandem PDZ3 domain protein (PDZ3-3) on CFTR channel activ- (L1480A) in CFTR, which abolishes the binding to CAP70, completely eliminated the CAP70-mediated po- ity.
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ABCC7 p.Leu1480Ala 11051556:212:161
status: NEW278 The CFTR-L1480A was derived bind to the C terminus of CFTR (Hall et al., 1998; Short from the pRSV-CFTR by replacing the HpaI/ApaI fragment with a et al., 1998; Wang et al., 1998).
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ABCC7 p.Leu1480Ala 11051556:278:9
status: NEW