ABCMdb

PMID: 11051556

Wang S, Yue H, Derin RB, Guggino WB, Li M
Accessory protein facilitated CFTR-CFTR interaction, a molecular mechanism to potentiate the chloride channel activity.
Cell. 2000 Sep 29;103(1):169-79., [PubMed]

Sentences

No. Mutations Sentence Comment

113 ABCC7 p.Leu1480Ala The amino acid substitution of leucine with alanine at the C-terminal end (L1480A) was designated as HA-CFTR-Ct*. Login to comment 157 ABCC7 p.Leu1480Ala ABCC7 p.Leu1480Ala (f and g) Open probability (Po) and mean opening time (MOT) of the wild-type CFTR and mutated CFTR-L1480A are plotted against different concentrations of purified recombinant CAP70 protein: filled squares, wild-type CFTR plus CAP70; filled circles, CFTR (L1480A) plus CAP70; open circles, wild-type CFTR plus PDZ3-3. Login to comment 159 ABCC7 p.Leu1480Ala n for wild-type CFTR is 5d51;9, for CFTR/L1480A is 4 and for PDZ3-3 is 5. Login to comment 212 ABCC7 p.Leu1480Ala substitution of the last residue leucine with alanine these possibilities, we tested the effect of a tandem PDZ3 domain protein (PDZ3-3) on CFTR channel activ- (L1480A) in CFTR, which abolishes the binding to CAP70, completely eliminated the CAP70-mediated po- ity. Login to comment 278 ABCC7 p.Leu1480Ala The CFTR-L1480A was derived bind to the C terminus of CFTR (Hall et al., 1998; Short from the pRSV-CFTR by replacing the HpaI/ApaI fragment with a et al., 1998; Wang et al., 1998). Login to comment