ABCMdb

PMID: 11158634

Raghuram V, Mak DO, Foskett JK
Regulation of cystic fibrosis transmembrane conductance regulator single-channel gating by bivalent PDZ-domain-mediated interaction.
Proc Natl Acad Sci U S A. 2001 Jan 30;98(3):1300-5. Epub 2001 Jan 23., 2001-01-30 [PubMed]

Sentences

No. Mutations Sentence Comment

61 ABCC7 p.Leu1480Ala ABCC7 p.Leu1480Ala [35 S]methionine-labeled full-length CFTR and CFTR-L1480A (Leu-1480 was replaced by Ala) was prepared by using an SP6 TnT (Promega) kit in the presence of canine pancreatic microsomes (Promega). Login to comment 110 ABCC7 p.Leu1480Ala ABCC7 p.Leu1480Val ABCC7 p.Leu1480Met The mutation L1480A resulted in a complete loss of interaction, and mutations T1378A, L1480V, and L1480M resulted in markedly reduced interaction (Fig. 1D), with both PDZ1 and PDZ2 having similar specificities for their target ligands. Login to comment 112 ABCC7 p.Leu1480Ala However, there are no additional PDZ binding sites in CFTR, because the PDZ1-2 domains bound to full-length in vitro-translated CFTR but not to mutant CFTR (L1480A) (Fig. 1C). Login to comment 142 ABCC7 p.Leu1480Ala [35S]methionine-labeled in vitro-translated products (I) and the GST-PDZ1-2 (1-299) ''pull-down`` products (P) of wild-type CFTR (WT) and mutant CFTR (L1480A) are indicated. Login to comment