ABCMdb

ABCC7 p.Arg352Glu

None has been submitted yet.

Publications

PMID: 10220340 [PubMed] Guinamard R et al: "Arg352 is a major determinant of charge selectivity in the cystic fibrosis transmembrane conductance regulator chloride channel."

No. Sentence Comment

166 Unfortunately, the single-channel conductance and kinetics of the R352E mutant appeared to be too small to accurately determine the reversal potential. Login to comment

PMID: 17043152 [PubMed] Aubin CN et al: "Positive charges at the intracellular mouth of the pore regulate anion conduction in the CFTR chloride channel."

No. Sentence Comment

7 However, the very low conductance of both R303E and R352E-CFTR could be greatly increased by elevating intracellular Cl-concentration. Login to comment
43 In contrast to wild-type CFTR, mutants R303E and, to a lesser extent, R352E showed outward rectification of the I-V relationship with symmetrical 154 mM Cl- solutions. Login to comment
63 In contrast, both R303E and R352E were associated with strong outward rectification (Fig. 2 A), which is quantified in Fig. 2 B. Login to comment
78 Unfortunately, because of low current density in this mutant, R352E could not be studied at low Cl-concentration. Login to comment
95 (B) Mean single channel current-voltage relationships constructed from such recordings for wild type (■), R303E (●) and R352E (○). Login to comment
100 Single channel currents carried by R303E and R352E under the same symmetrical Cl- solutions used in Fig. 2 are shown in Fig. 5 A. Login to comment
103 For example, at -80 mV, single channel current amplitude was reduced by 89 ± 0% (n = 4) in R303E and by 75 ± 2% (n = 3) in R352E, while at +80 mV, unitary currents were reduced by 30 ± 1% (n = 4) in R303E and by 25 ± 1% (n = 4) in R352E (Fig. 5 B). Login to comment
104 The Cl-dependence of I-V shape in R303E and R352E is consistent with removal of positive surface charges that normally act to increase the Cl-concentration at the inner mouth of the pore. Login to comment
114 (A) Example single channel currents recorded from wild type, R303E, and R352E at different intracellular Cl- concentrations (154 or 304 mM, as indicated), at a membrane potential of -20 mV. For each trace, the line to the left represents the current level when the channel is closed. Login to comment
149 Under these conditions, current carried not only by wild-type CFTR, but also by each of the channel mutants R303E, R352E (Fig. 10), R80E, R242E, R933E, R1102E, and R352Q (not depicted) showed reversal potentials that were not significantly different from the calculated Cl- equilibrium potential (+33.4 mV). Login to comment
184 Furthermore, rectification in R303Q and R303E appeared more sensitive to the intracellular Cl-concentration than in R352Q and R352E (Fig. 3 B). Login to comment
196 Unfortunately we were unable to obtain single channel recordings at Cl- concentrations higher than 304 mM; however, the dramatic increase in conductance seen in both R303E and R352E when intracellular [Cl-] was increased from 154 to 304 mM (Fig. 6) suggests that the conductance of these mutant channels is far from saturated at this elevated Cl-concentration, consistent with a large increase in Km. Login to comment
199 Nevertheless, unitary currents carried by Cl- influx were also reduced in both R303E and R352E (Fig. 5). Login to comment

PMID: 18421494 [PubMed] Cui G et al: "Mutations at arginine 352 alter the pore architecture of CFTR."

No. Sentence Comment

6 Full conductance state amplitude was similar to that of wild-type CFTR in all mutants except R352E, suggesting that R352 does not itself form an anion coordination site. Login to comment
8 Wild-type-like properties were rescued in R352E/D993R-CFTR, suggesting that R352 and D993 in the wild-type channel may interact to stabilize pore architecture. Login to comment
9 Finally, R352A-CFTR was sensitive to modification by externally applied MTSEA+ , while wild-type and R352E/D993R-CFTR were not. Login to comment
137 Both f state slope conductances were significantly reduced in R352E-CFTR (Table 1). Login to comment
139 The single-channel conductance of the f state exhibited significant outward rectification in R352A-, R352Q- and R352E-CFTR (Table 1). Login to comment
146 Anion Selectivity of R352A-, R352E- and R352K-CFTR To determine whether mutations at R352 affected the ability of CFTR channels to select between ions of similar charge, we studied the anion selectivity patterns of R352A-, R352E- and R352K-CFTR using inside-out macropatches and compared them to WT-CFTR. Login to comment
152 For calculation of Gx/GCl, we compared R352A-, R352E- and R352K-CFTR with WT-CFTR as well as R352E- and R352K-CFTR with R352A-CFTR. Login to comment
162 Slope conductances are summarized in Table 1 Table 1 Slope conductancea (in pS) of the f state of WT-CFTR and multiple single and double mutants CFTR n Negative VM Positive VM WT 7 6.82 ± 0.03 6.97 ± 0.06 R352A 6 6.80 ± 0.06 7.85 ± 0.07*, ** R352Q 6 5.29 ± 0.02* 6.28 ± 0.05*, ** R352K 5 6.87 ± 0.03 6.86 ± 0.01 R352E 5 3.78 ± 0.01* 6.03 ± 0.01*, ** R352E/E873R 6 3.84 ± 0.01* 5.64 ± 0.01*, ** R352E/ E1104R 6 4.36 ± 0.01* 5.86 ± 0.02*, ** R352E/D993R 5 5.90 ± 0.02* 6.44 ± 0.01*, ** D993R 7 8.27 ± 0.05* 7.13 ± 0.07** a Slope conductance indicates single-channel conductance calculated from 0 to +100 mV (positive VM) or to -100 mV (negative VM) by linear regression * P B 0.001 compared to the equivalent slope conductance in WT-CFTR, ** P B 0.001 compared to the slope conductance in the same mutant at negative VM reflects the loss of anion binding properties within the core of the permeation pathway, which contributes to the tight binding of SCN (Smith et al. 1999). Login to comment
166 Our present results suggest -300 -50 300 50 Br -100 100 NO3 Cl SCN pA mVBr NO3 SCN Cl -300 -50 300 50 Br -100 100 NO3 Cl SCNC pA mVBr NO3 SCN Cl R352E -4000 -50 4000 50 -100 100 -800 -50 800 50 -100 100 -6000 -50 6000 50 -100 100 A pA -50 800 50 -100 100 A SCN Br Cl NO3 NO3 Br Cl SCN Br NO3 SCN Cl Br NO3 SCN Cl -800 mV pA mV pA mV pA mV NO3 Br Cl SCN Br NO3 SCN Cl Br NO3 SCN Cl Br NO3 SCN Cl -4000 -50 4000 50 -100 100 D -800 -50 800 50 -100 100 E D993R -6000 -50 6000 50 -100 100 WT pA -50 800 50 -100 100 B SCN Br Cl NO3 NO3 Br Cl SCN Br NO3 SCN Cl Br NO3 SCN Cl -800 mV pA mV pA mV pA mV NO3 Br Cl SCN Br NO3 SCN Cl Br NO3 SCN Cl Br NO3 SCN Cl R352A R352K R352E/ Fig. 5 Mutations at R352 alter anion selectivity. Login to comment
167 Representative inside-out macropatches, recorded in the presence of cytoplasmic Cl- or Cl- plus substitute anions, with voltage ramps between -100 and +100 mV, are shown for (A) WT-CFTR, (B) R352A-CFTR, (C) R352E-CFTR, (D) R352K-CFTR and (E) the double mutant R352E/D993R-CFTR. Login to comment
171 Solutions were at pH 7.45 and are labeled as follows: 150 mM Cl- (black), 130 mM Cl- plus 20 mM NO3 - (purple), 130 mM Cl- plus 20 mM Br- (green) and 130 mM Cl- plus 20 mM SCN- (red) Table 2 Relative permeabilities of some anions in WT-CFTR and R352-CFTR mutants * Significant difference compared with WT-CFTR, P \ 0.05; ** Significant difference compared with R352A, P \ 0.05 CFTR n SCN Br NO3 WT 6 4.11 ± 0.17 1.45 ± 0.04 1.51 ± 0.02 R352A 10 4.18 ± 0.65 1.35 ± 0.21 1.70 ± 0.29 R352E 6 5.18 ± 0.32* 1.47 ± 0.08 1.64 ± 0.43 R352K 7 4.05 ± 0.12 1.52 ± 0.01 1.59 ± 0.03** R352E/D993R 6 3.62 ± 0.06* 1.48 ± 0.04 1.59 ± 0.02** Table 3 Relative conductances of some anions in WT-CFTR and R352-CFTR mutants CFTR n SCN Br NO3 WT 6 0.16 ± 0.02 0.67 ± 0.04 0.84 ± 0.04 R352A 10 1.59 ± 0.12* 1.31 ± 0.08* 1.59 ± 0.14* R352E 6 2.73 ± 0.31*, ** 1.49 ± 0.22* 1.54 ± 0.12* R352K 7 1.12 ± 0.08*, ** 0.99 ± 0.02*, ** 1.73 ± 0.26* R352E/ D993R 7 0.61 ± 0.05*, ** 0.98 ± 0.03*, ** 1.26 ± 0.13* Relative conductance was measured at VM = Vrev -25 mV * Significant difference compared with WT-CFTR, P\0.05; ** Significant difference compared with R352A, P\0.05 that loss of positive charge at position 352 destroyed the overall pore architecture, which subsequently changed the anion selectivity characteristics as seen in R352A- and R352E-CFTR. Login to comment
173 Furthermore, the finding that relative permeability values are nearly identical in R352A-, R352E- and R352K-CFTR suggests that the role of this site in determining anion selectivity is only indirect. Login to comment
186 In summary, mutations at R352 that destroyed the positive charge (R352A, R352E and R352Q) altered the pore architecture of CFTR and caused instability of the open state, changing anion selectivity and pore block by glipizide. Login to comment
198 To identify the interaction partner for R352, we replaced R352 with an acidic residue (R352E) and introduced an arginine residue in the place of candidate interaction partners. Login to comment
199 We studied the conductance properties of CFTR channels bearing the following mutations: R352E, R352E/E873R, R352E/ D993R and R352E/E1104R. Login to comment
201 Three of these mutants, R352E-, R352E/E873R- and R352E/E1104R-CFTR, exhibited instability of the open state, in which the amplitudes of the s1, s2 and f conductance states were very similar between the three mutants. Login to comment
202 R352E/D993R-CFTR, in contrast, exhibited stability of the full conductance state similar to that seen in WT-CFTR and R352K-CFTR (Fig. 1); transitions to the s1 and s2 states were rare events in this double mutant. Login to comment
204 R352E-, R352E/ E873R- and R352E/E1104R-CFTR exhibited significant outward rectification, while WT-CFTR did not (Table 1). Login to comment
205 The slope conductance of R352E/D993R-CFTR was slightly lower than that of WT-CFTR, although linearity of the i-V relation was mostly retained. Login to comment
206 These data suggested that the D993R mutation at least partly compensated for the R352E mutation, although the double mutant R352E/ D993R-CFTR did not fully recapitulate the behavior of WT-CFTR. Login to comment
208 If D993 served as the interaction partner of R352, we would expect that block of R352E/D993R-CFTR would be similar to that 0.4 pA 2 s 0.4 pA 2 s 0.2 pA 2 s 0.2 pA 2 s c s1 s2 f c s1 s2 f c s1 s2 f c f R352E R352E/E873R R352E/E1104R R352E/D993R 0 4000 #ofevents 0.0 -0.4 -0.8 0.0 -0.4 -0.8 3000 #ofevents 0 #ofevents 0.0 -0.4 -0.8 3000 0 Currents (pA) 0.0 -0.4 0 2500#ofevents -0.8 fc s1 s2 s1 s2 s1 s2 0.4 pA 2 s 0.4 pA 2 s 0.2 pA 2 s 0.2 pA 2 s c s1 s2 f c s1 s2 f c s1 s2 f c f R352E R352E/E873R R352E/E1104R R352E/D993R 0.4 pA 2 s 0.4 pA 2 s 0.4 pA 2 s 0.4 pA 2 s 0.2 pA 2 s 0.2 pA 2 s 0.2 pA 2 s 0.2 pA 2 s c s1 s2 f c s1 s2 f c s1 s2 f c f R352E R352E/E873R R352E/E1104R R352E/D993R B C D A 0 4000 #ofevents 0.0 -0.4 -0.8 0 4000 #ofevents 0.0 -0.4 -0.8 0.0 -0.4 -0.8 3000 #ofevents 0 0.0 -0.4 -0.8 3000 #ofevents 0 #ofevents 0.0 -0.4 -0.8 3000 0 Currents (pA) #ofevents 0.0 -0.4 -0.8 3000 0 #ofevents 0.0 -0.4 -0.8 3000 0 Currents (pA) 0.0 -0.4 0 2500#ofevents -0.8 fc s1 s2 s1 s2 s1 s2 Fig. 7 Single-channel current tracings of R352E-CFTR and double mutants from excised inside-out patches (left) and resulting all-points amplitude histograms (right) under the same experimental conditions as in Fig. 1. Login to comment
210 There are four current levels indicating the c, s1, s2 and f states in all but the revertant mutant R352E/ D993R-CFTR, which only exhibited the c and f states. Login to comment
213 Figure 8B shows macropatch currents from R352E/D993R-CFTR in the presence and absence of 200 lM glipizide; time-dependent block was rescued in this double mutant. Login to comment
215 This result suggested that the revertant double mutation, R352E/ D993R, recovered the time-dependent block by glipizide but did not completely recover the sensitivity to glipizide characteristic of WT-CFTR. This may reflect the difference in side chain volumes between aspartic and glutamic acids; the volume of a glutamic acid side chain is 20% larger than that of an aspartic acid side chain (Creighton 1993), which may result in a different pore structure. Login to comment
216 To further explore the characteristics of R352E/D993R-CFTR, we studied anion selectivity between Cl- and four substitute monovalent anions. Login to comment
218 Overall, both relative permeability and relative conductance values for WTand R352E/ D993R-CFTR were similar (Tables 2, 3). Login to comment
219 R352E/D993R- CFTRcurrentsexhibitedthesameanionpermeabilitysequence as WT-CFTR: SCN- [NO3 - C Br- [Cl- (although PSCN/ PCl was clearly reduced in the double mutant). Login to comment
220 R352E/D993R-CFTR exhibited relative conductances to SCN- and Br- intermediate between that of WT-CFTR and R352A-CFTR (Table 3). Login to comment
221 These results also suggested that R352A-CFTR and R352E/D993R-CFTR have different pore architecture and that the selectivity properties of the pore of the double mutant might be slightly different from that of WT-CFTR. Login to comment
223 Because the data presented thus far suggested that D993 serves as the interacting partner of R352, we predicted that the D993R mutation alone would change channel activity in a manner similar to that of the R352E, -A or -Q mutation. Login to comment
232 Hence, it is likely that MTSEA+ modified one (or more) of the endogenous cysteines, which B WT-CFTR R352E/D993R R352E R352E/E873R R352E/E1104R WT-CFTR R352E/D993R R352E R352E/E873R R352E/E1104R mV -100 -50 50 100 -0.8 -0.4 0.4 0.8 pA 100 ms 0.2 nA A WT-CFTR R352E/D993R R352E R352E/E873R R352E/E1104R WT-CFTR R352E/D993R R352E R352E/E873R R352E/E1104R mV -100 -50 50 100 -0.8 -0.4 0.4 0.8 pA WT-CFTR R352E/D993R R352E R352E/E873R R352E/E1104R WT-CFTR R352E/D993R R352E R352E/E873R R352E/E1104R WT-CFTR R352E/D993R R352E R352E/E873R R352E/E1104R WT-CFTR R352E/D993R R352E R352E/E873R R352E/E1104R mV -100 -50 50 100 -0.8 -0.4 0.4 0.8 pA 100 ms 0.2 nA Fig. 8 The double mutant R352E/D993R-CFTR recovers WT-like channel behavior. Login to comment
233 (A) Single channel i-V relationships are shown for full conductance states of WT-, R352E-, R352E/E873R-, R352E/ E1104R- and R352E/D993R-CFTR. Login to comment
235 (B) Block of R352E/ D993R-CFTR macropatch currents by glipizide (200 lM) is time-dependent. Login to comment
239 If this were true, we would expect that the revertant double mutant, R352E/D993R-CFTR, would not respond to MTSEA+ . Login to comment
244 In contrast, R352E/ D993R-CFTR was insensitive to exposure to MTSEA+ , which resulted in only a 1.08 ± 0.02-fold increase in current (n = 6), thus indicating that the double mutant exhibited WT-like behavior. Login to comment
246 First, channels bearing charge-destroying mutations at this site, including R352Q, R352E and R352A, exhibited instability of the open state compared to WT-CFTR, as indicated by frequent transitions between all three open conductance states (s1, s2, f). Login to comment
253 In c, points show mean ± SEM for n = 7 observations, and error bars are smaller than the symbols; lines are from linear regression WT 1 A 200 s Isoproterenol 0.4 A 100 s 0.4 A 100 s R352A R352E/D993R MTSEA MTSEA MTSEA WT 1 A 200 s Isoproterenol 0.4 A 100 s 0.4 A 100 s R352A R352E/D993R MTSEA MTSEA MTSEA Fig. 10 Mutation R352A results in appearance of sensitivity to a cysteine-modifying reagent. Login to comment
254 Oocytes expressing WT-CFTR (top trace), R352A-CFTR (middle trace) or R352E/D993R-CFTR (bottom trace), along with the b2-adrenergic receptor, were studied by two-electrode voltage clamp. Login to comment
258 Second, we identified the interaction partner as D993 by use of double mutants; R352E/E873R-CFTR and R352E/E1104R-CFTR exhibited permeation properties similar to those of R352E-CFTR, while R352E/D993R-CFTR behaved more like WT-CFTR. Login to comment
261 As predicted, D993R-CFTR exhibited instability of the open state similar to that seen in R352E-CFTR. Login to comment
295 Stability of the open state was retained in the case of a charge-conserving mutation, R352K, and in the double mutant R352E/D993R-CFTR. Login to comment
297 Compared to WT-CFTR, R352E/D993R-CFTR channels exhibited lower slope conductance, weakened block by glipizide, and altered selectivity between Cl- and SCN- . Login to comment
300 We also note that while the relative conductance values for SCN- , Brand NO3 - are shifted in the same direction in R352K-CFTR as they are in R352A- or R352E-CFTR, the shifts for SCN- and Br- are smaller in R352K-CFTR than in the charge-destroying mutants. Login to comment
301 We conclude that the double mutant R352E/D993R-CFTR retains the interaction between these residues but does not fully mimic the behavior of WT-CFTR, suggesting that permeation properties in the CFTR chloride channel are very sensitive to small changes in pore structure. Login to comment

PMID: 19020075 [PubMed] Jordan IK et al: "Evolutionary and functional divergence between the cystic fibrosis transmembrane conductance regulator and related ATP-binding cassette transporters."

No. Sentence Comment

95 Isolated bursts of channel activity from oocytes expressing WT-CFTR, R352E-CFTR, R352E/E1104R-CFTR, and R352E/D993R-CFTR. Login to comment
99 Channels bearing the R352E mutation, or the double mutant R352E/E1104R, exhibited frequent transitions to subconductance levels. Login to comment
100 In contrast, WT-CFTR channels, and channels bearing the revertant mutation R352E/D993R, primarily exhibit transitions to the full conductance level. Login to comment
131 In contrast, approximately wild-type channel behavior is retained in R352K-CFTR and the charge-swapping double mutant, R352E/D993R-CFTR (Fig. 3). Login to comment

PMID: 22160394 [PubMed] Cui G et al: "Differential contribution of TM6 and TM12 to the pore of CFTR identified by three sulfonylurea-based blockers."

No. Sentence Comment

144 These functional characteristics were returned to approximately their wildtype behavior in R352E/D993R-CFTR, perhaps because the salt bridge was retained in this second-site reversion [12]. Login to comment

PMID: 23709221 [PubMed] Cui G et al: "Two salt bridges differentially contribute to the maintenance of cystic fibrosis transmembrane conductance regulator (CFTR) channel function."

No. Sentence Comment

104 In our prior studies, we found that R352E-CFTR can open to all three conductance levels, with all open states being unstable. Login to comment
105 Similar results were found for D993R-CFTR, but nearly wild type-like behavior, including stable openings to the f state, was recovered in the R352E/D993R double mutant (see Fig. 3A). Login to comment
109 We therefore hypothesized that Arg347 might also interact with Asp993 to rescue the CFTR channel pore to a stable f state and tested this hypothesis in three double mutants; TABLE 1 Summary of the effects of mutations studied Mutant Main features of open bursts Impact on f state R347A Emphasizes s1 state, brief transitions to s2 and f Can reach f but not stable R347D Emphasizes s1 state, no transitions to s2 and f Cannot reach f D924R Brief transitions to all conductance levels Can reach f but not stable R347K Wild type-like Wild type-like R347D/D924R Emphasizes s2 state, rare and brief transitions to f Can reach f but not stable R352E Opens to all 3 levels; s1 much more stable than in WT, s2 unstable, f unstable Can reach f but not stable D993R Opens to all 3 levels, but none are stable Can reach f but not stable R352E/D993R Wild type-like, with increased transitions to s1 and s2; slightly reduced single-channel conductance Wild type-like R352E/D924R Opens to all 3 levels, but none are stable Can reach f but not stable R347D/D993R Very stable s2; rare and brief transitions to both s1 and f Can reach f but not stable R347A/R352A Opens to all 3 levels; s1 much more stable than in WT, s2 unstable, f unstable Can reach f but not stable R347D/D924R/D993R Opens to all 3 levels; s1 much more stable than in WT, s2 relatively stabilized, f unstable Can reach f but not stable R347D/D924R/R352E/D993R Primarily flickers between s2 and f; s1 much more stable than in WT, slightly reduced single channel conductance Can reach f but not stable FIGURE 3. Login to comment
111 A, representative current samples of R352E/D993R-, R352E/D924R-, and R347D/D993R-CFTR recorded from excised inside-out patches with the same conditions as Fig. 2 (n afd; 3-6 for each mutant). Login to comment
114 As noted above, R352E/D993R exhibited a prominent full open state similar to WT-CFTR (13), suggesting that the R352E/D993R salt bridge can fully rescue the CFTR channel pore to normal behavior (aside from a slight decrease in single channel conductance). Login to comment
115 Whereas the single channel behavior of R352E/D924R was similar to that of R352E alone, with multiple unstable open states, suggesting that Arg352 and Asp924 do not interact, R347D/D993R was much more like R347D/D924R, with the s2 state dominant (compare Figs. 3 and 2). Login to comment
141 However, the quadruple mutant R347D/D924R/D993R/R352E did not completely rescue WT behavior (Fig. 4, A and B). Login to comment
146 Representative current samples of R347A/R352A-, R347D/D924R/D993R-, and R347D/D924R/D993R/R352E-CFTR were recorded under the same conditions as in Fig. 3 (n afd; 5-6 for each mutant) (A). Login to comment
162 Recovery of Charge at R352C and D993C Rescued Channel Stability in the Full Open State-R352C-CFTR exhibited single channel behavior similar to that previously reported for R352A-, R352Q-, and R352E-CFTR (13). Login to comment

PMID: 23784545 [PubMed] Cai Z et al: "Acute inhibition of the cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channel by thyroid hormones involves multiple mechanisms."

No. Sentence Comment

251 The occurrence of subconductance states is more frequent in some site-directed mutations in the MSDs [e.g., R334C-CFTR (70), R347E-CFTR (11), and R352E-CFTR (13)], while wild-type murine CFTR resides for prolonged periods in a minuscule subconductance state and only transitions infrequently to the full open-state (37). Login to comment