ABCC1 p.Trp222Leu
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PMID: 16098482
[PubMed]
Noguchi T et al: "MRP1 mutated in the L0 region transports SN-38 but not leukotriene C4 or estradiol-17 (beta-D-glucuronate)."
No.
Sentence
Comment
4
In this paper, we further tested the effect of W222L, W223L and R230A mutations in MRP1, designated dmL0MRP1, on MRP1 transport activity.
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ABCC1 p.Trp222Leu 16098482:4:47
status: NEW61 The extra mutations created, W222L, W223L and R230A, were located within WxxxxxK sequences in the L0 region that have been reported to an interacting site of glutathione-S-transferase with GSH.
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ABCC1 p.Trp222Leu 16098482:61:29
status: NEW64 W222L, W223L and R230A mutations were then introduced into this construct by PCR using the forward primers, 50 -GGGTTGATTGTCGCGGGCTACCGCC-30 , and reverse primer 50 -TGTGATCAACAAGAAGGTGATCC- TC-30 (bold denotes mismatched bases encoding the mutation).
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ABCC1 p.Trp222Leu 16098482:64:0
status: NEW136 As described in Section 2, the new mutant MRP1, named dmL0MRP1, was designed to carry the extra mutations, W222L, W223L and R230A, in addition to the previous mutants, W261A and K267M, in L0 region, and the original TMD0 region, in order to avoid the influence of deletion of the TMD0 region (Fig. 1).
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ABCC1 p.Trp222Leu 16098482:136:107
status: NEW
PMID: 17187268
[PubMed]
Bakos E et al: "Portrait of multifaceted transporter, the multidrug resistance-associated protein 1 (MRP1/ABCC1)."
No.
Sentence
Comment
97
Typical substrates, which are transported as glutathione or glucuronide conjugates, e.g., LTC4 or E217βG, are not transported by an MRP1 variant mutated in the L0 region (W222L, W223L, R231A, W261A, K267M).
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ABCC1 p.Trp222Leu 17187268:97:177
status: NEW
PMID: 21143116
[PubMed]
He SM et al: "Structural and functional properties of human multidrug resistance protein 1 (MRP1/ABCC1)."
No.
Sentence
Comment
720
Membrane vesicles prepared from cells expressing mutated MRP1/ABCC1 at Trp222Leu, Trp223Leu, or Arg230Ala within L0 could transport SN-38 (active metabolite of irinotecan), but not LTC4 or E217 G which are transported by MRP1/ABCC1 in a GSH-dependent manner, and could not be photolabeled with 11-azidophenyl agosterol A [279].
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ABCC1 p.Trp222Leu 21143116:720:71
status: NEW