ABCMdb

PMID: 9788623

Russ G, Ramachandra M, Hrycyna CA, Gottesman MM, Pastan I, Bennink JR, Yewdell JW
P-glycoprotein plays an insignificant role in the presentation of antigenic peptides to CD8+ T cells.
Cancer Res. 1998 Oct 15;58(20):4688-93., [PubMed]

Sentences

No. Mutations Sentence Comment

64 ABCB1 p.Asp555Asn ABCB1 p.Asp555Asn ABCB1 p.Asp1200Asn ABCB1 p.Asp1200Asn Further rVVs were used to express P-glycoprotein |MDRI(T7)] and P-glycoprotein mutants (NM- D555N, CM-D1200N, and DM-D555N + D1200N), under the control of the T7 promoter (14). Login to comment 67 ABCB1 p.Asp555Asn ABCB1 p.Asp555Asn ABCB1 p.Asp1200Asn ABCB1 p.Asp1200Asn Further rVVs were used to express P-glycoprotein |MDRI(T7)] and P-glycoprotein mutants (NM- D555N, CM-D1200N, and DM-D555N + D1200N), under the control of the T7 promoter (14). Login to comment 105 ABCB1 p.Asp555Asn ABCB1 p.Asp1200Asn To determine the requirement for a functional P-glycoprotein, we used rVVs expressing three mutated forms of the protein under the control of the T7 promoter, termed CM, NM, and DM. The introduced mutations alter one or both of highly conserved Asp residues located, respectively, within Walker B regions of either NH2-terminal (D555N) or COOH-terminal (D1200N) ATP binding/utilization sites that are believed to be involved in binding to Mg2+. Login to comment 109 ABCB1 p.Asp555Asn ABCB1 p.Asp1200Asn The introduced mutations alter one or both of highly conserved Asp residues located, respectively, within Walker B regions of either NH2-terminal (D555N) or COOH-terminal (D1200N) ATP binding/utilization sites that are believed to be involved in binding to Mg2+. Login to comment