ABCMdb

PMID: 9175873

Annereau JP, Wulbrand U, Vankeerberghen A, Cuppens H, Bontems F, Tummler B, Cassiman JJ, Stoven V
A novel model for the first nucleotide binding domain of the cystic fibrosis transmembrane conductance regulator.
FEBS Lett. 1997 May 5;407(3):303-8., [PubMed]

Sentences

No. Mutations Sentence Comment

70 ABCC7 p.Arg766Met ABCC7 p.Asp648Val ABCC7 p.Gly628Arg ABCC7 p.Thr665Ser ABCC7 p.Leu610Ser ABCC7 p.Leu633Pro The maturation patterns of six mutant R domain proteins were determined (Fig. 3): CFTR-L610S, CFTR-G628R and CFTR-L633P matured to the core-glycosylated form, while CFTR-D648V, CFTR-T665S and CFTR-R766M matured to the complete glycosylated form. Login to comment 134 ABCC7 p.Arg766Met ABCC7 p.Leu610Ser COS cells transfected with wild-type CFTR-L610S and CFTR-R766M were metabolically labelled, chased and CFTR was immunoprecipitated and separated on a SDS-PAGE gel. Login to comment 135 ABCC7 p.Arg766Met ABCC7 p.Leu610Ser COS cells transfected with wild-type CFTR-L610S and CFTR-R766M were metabolically labelled, chased and CFTR was immunoprecipitated and separated on a SDS-PAGE gel. Login to comment 150 ABCC7 p.Arg766Met ABCC7 p.Asp648Val ABCC7 p.Gly628Arg ABCC7 p.Thr665Ser ABCC7 p.Leu610Ser ABCC7 p.Leu633Pro The mutations L610S (tc at 1961), G628R (gc at 2014), L633P (tc at 2030), D648V (at at 2075), T665S (at at 2125) and R766M (gt at 2429) (nucleotide and amino acid assignment according to [2]) were introduced using the Transformer Site-Directed Mutagenesis kit (Clontech, Heidelberg, Germany). Login to comment 151 ABCC7 p.Arg766Met ABCC7 p.Asp648Val ABCC7 p.Gly628Arg ABCC7 p.Thr665Ser ABCC7 p.Leu610Ser ABCC7 p.Leu633Pro The mutations L610S (tc at 1961), G628R (gc at 2014), L633P (tc at 2030), D648V (at at 2075), T665S (at at 2125) and R766M (gt at 2429) (nucleotide and amino acid assignment according to [2]) were introduced using the Transformer Site-Directed Mutagenesis kit (Clontech, Heidelberg, Germany). Login to comment